Summary
International Symposium on Nonlinear Theory and its Applications
2009
Session Number:B1L-A
Session:
Number:B1L-A3
Microcantilever-based Biosensor using Frequency Modulation Detection Method in Liquids
Kei Kobayashi, Yu Babasaki, Yotaro Kobayashi, Kei Noda, Hirofumi Yamada, Kazumi Matsushige,
pp.-
Publication Date:2009/10/18
Online ISSN:2188-5079
Summary:
We have developed a microcantilever-based biosensor using the frequency modulation (FM) detection method in liquids, which is capable of detecting the resonance frequency shift of the cantilever induced by the adsorption of the biomolecules on the cantilever. Since we found that the frequency fluctuation of the cantilever is strongly dependent on the noise-equivalent displacement in the displacement sensor, we decreased the sensor noise down to the level which is close to the shot noise limit. We employed an intensity-modulated laser light source to excite the cantilever oscillation, instead of using the piezoelectric actuator in order to avoid excitation of the mechanical resonances of the liquid cell. We successfully detected the adsorption of the DNA oligomer molecules on the gold-coated cantilever using the FM detection method.