Summary

Proceedings of the 2012 International Symposium on Nonlinear Theory and its Applications

2012

Session Number:A1L-B

Session:

Number:37

Quantifying viral dynamics of highly and less pathogenic simian/human immunodeficiency viruses from in vitro experimental data

Shingo Iwami,  

pp.37-40

Publication Date:

Online ISSN:2188-5079

DOI:10.15248/proc.1.37

PDF download (707.7KB)

When virologists have interests in the kinetics of virus replication, they perform certain experiments using cell cultures. To reveal the features of virus replication, certain parameters (e.g., the levels of viral components and activities) at certain time points are usually measured. The obtained data, which we call it “snap-shot” data, might reflect one of the aspects of virus replication. However, virus infection is the complex phenomenon that is consisted of the consecutive interactions with viruses, their target cells, and infected cells. Therefore, it would be difficult to elucidate the overall features of dynamic and complex phenomenon based on “snap-shot” data. In order to completely decompose and quantify the dynamics of virus infection, combining mathematical modeling, mathematical analysis, and numerical simulation with the experimental data is a powerful way. Here we introduce a method to “quantitatively” investigate the dynamics of virus infection in in vitro cell culture and discuss the potential of the combinational analyses with experimental and computational virology for understanding highly and less viral pathogenesis.

[1] Harouse JM, Gettie A, Tan RC, Blanchard J, and Cheng-Mayer C: Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs. Science 1999, 284:816-819.

[2] Matsuda K, Inaba K, Fukazawa Y, Matsuyama M, Ibuki K, Horiike M, Saito N, Hayami M, Igarashi T, and Miura T: In vivo analysis of a new R5 tropic SHIV generated from the highly pathogenic SHIV-KS661, a derivative of SHIV-89.6. Virology 2010, 399:134-143.

[3] Nishimura Y, Igarashi T, Donau OK, Buckler-White A, Buckler C, Lafont BA, Goeken RM, Goldstein S, Hirsch VM, and Martin MA: Highly pathogenic SHIVs and SIVs target different CD4⁺ T cell subsets in rhesus monkeys explaining their divergent clinical courses. Proc. Natl. Acad. Sci. USA 2004, 101:12324-12329.

[4] Reimann KA, Li JT, Veazey R, Halloran M, Park IW, Karlsson GB, Sodroski J, and Letvin NL: A chimeric simian/human immunodeficiency virus expressing a primary patient human immunodeficiency virus type 1 isolate env causes an AIDS-like disease after in vivo passage in rhesus monkeys. J. Virol. 1996, 70:6922-6928.

[5] Shinohara K, Sakai K, Ando S, Ami Y, Yoshino N, Takahashi E, Someya K, Suzaki Y, Nakasone T, Sasaki Y, Kaizu M, Lu Y, and Honda M: A highly pathogenic simian/human immunodeficiency virus with genetic changes in cynomolgus monkey. J. Gen. Virol. 1999, 80:1231-1240.

[6] Kozyrev IL, Ibuki K, Shimada T, Kuwata T, Takemura T, Hayami M, and Miura T: Characterization of less pathogenic infectious molecular clones derived from acute-pathogenic SHIV-89.6p stock virus. Virology 2001, 282:6-13.

[7] Miyake A, Ibuki K, Enose Y, Suzuki H, Horiuchi R, Motohara M, Saito N, Nakasone T, Honda M, Watanabe T, Miura T, and Hayami M: Rapid dissemination of a pathogenic simian/human immunodeficiency virus to systemic organs and active replication in lymphoid tissues following intrarectal infection. J. Gen. Virol. 2006, 13:11-20.

[8] Inaba K, Fukazawa Y, Matsuda K, Himeno A, Matsuyama M, Ibuki K, Miura Y, Koyanagi Y, Nakajima A, Blumberg RS, Takahashi H, Hayami M, Igarashi T, and Miura T: Small intestine CD4⁺ cell reduction and enteropathy in simian/human immunodeficiency virus KS661-infected rhesus macaques in the presence of low viral load. J. Gen. Virol. 2010, 91:773-781.

[9] Iwami S, Holder BP, Beauchemin CAA, Morita S, Tada T, Sato K, Igarashi T, and Miura T: Quantification system for the viral dynamics of a highly pathogenic simian/human immunodeficiency virus based on in vitro experiment and a mathematical model. Retrovirology. 2012, 9: 18.

[10] Nowak MA and May RM: Virus dynamics, Oxford University Press; 2000.

[11] Perelson AS: Modeling viral and immune system dynamics. Nat. Rev. Immunol. 2001, 2: 28-36.

[12] Beauchemin CA, McSharry JJ, Drusano GL, Nguyen JT, Went GT, Ribeiro RM, and Perelson AS: Modeling amantadine treatment of influenza A virus in vitro. J. Theor. Biol. 2008, 254:439-451.