| Presentation | 2015-03-16 Development and evaluation of micro cell culture wells for studies on efficient sonoporation You SHIRAHATA, (KURIBAYASHI) Kaori SHIGETOMI, Nobuki KUDO, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have been studying a sonoporation technique that transduces drugs into cells by exposure of the cells with adjacent bubbles to short-pulsed ultrasound. In the research process, it was found that efficiency of the cell membrane repair after sonoporation is affected by the cell membrane tension. In this study, micron-sized wells that can culture several tens of cells were designed and fabricated to control cell membrane tension by changing extension state of the cells. The wells were manufactured using a conventional photolithography technique with thick photoresist SU-8. The wells are designed to be 15-200 μm in diameter and about 40 μm in depth. The developed wells were used for 1-week cell culture, and it was confirmed that adherent areas of cells can be controlled by controlling number of the cells cultured inside a well, indicating that the developed wells are useful tools for future studies on in vivo sonoporation. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | sonoporation / ultrasound / microbubble / photolithography / SU-8 |
| Paper # | MBE2014-119,NC2014-70 |
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| Conference Information | |
| Committee | NC |
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| Conference Date | 2015/3/9(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Neurocomputing (NC) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Development and evaluation of micro cell culture wells for studies on efficient sonoporation |
| Sub Title (in English) | |
| Keyword(1) | sonoporation |
| Keyword(2) | ultrasound |
| Keyword(3) | microbubble |
| Keyword(4) | photolithography |
| Keyword(5) | SU-8 |
| 1st Author's Name | You SHIRAHATA |
| 1st Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University() |
| 2nd Author's Name | (KURIBAYASHI) Kaori SHIGETOMI |
| 2nd Author's Affiliation | Faculty of Health Sciences, Hokkaido University |
| 3rd Author's Name | Nobuki KUDO |
| 3rd Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University |
| Date | 2015-03-16 |
| Paper # | MBE2014-119,NC2014-70 |
| Volume (vol) | vol.114 |
| Number (no) | 515 |
| Page | pp.pp.- |
| #Pages | 5 |
| Date of Issue | |