| Presentation | 2014-07-11 Development of Digital Cell Manipulation Station for Massively Parallel Analysis of Single Cells Moeto NAGAI, Yuya MATSUSE, Satoshi SOGA, Sho KAWAHARADA, Takahiro KAWASHIMA, Takayuki SHIBATA, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | This study aims to place single cells in an array format for massively parallel analysis of cellular functions. Cell manipulation system is composed of a cell feeder, micromanipulator array, and microwell array. Bidirectional electrosmotic pumps, driven by DC-biased AC voltages, were used to manipulate cells. We applied voltage on surrounding comb electrodes of a cell feeder and generated a flow toward a central microwell array. In the central region, a dielectrophoretic force was used to trap single cells in a microwell. A manipulator array was fabricated by connecting a micronozzle array with a pump. We demonstrate suction of cells into nozzle holes using the manipulator array. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Cell Manipulation / High-Throughput Analysis / Cell Feeder / Manipulator Array / Microwell Array / Electrokinetic Forces |
| Paper # | ED2014-51 |
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| Conference Information | |
| Committee | ED |
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| Conference Date | 2014/7/3(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Electron Devices (ED) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Development of Digital Cell Manipulation Station for Massively Parallel Analysis of Single Cells |
| Sub Title (in English) | |
| Keyword(1) | Cell Manipulation |
| Keyword(2) | High-Throughput Analysis |
| Keyword(3) | Cell Feeder |
| Keyword(4) | Manipulator Array |
| Keyword(5) | Microwell Array |
| Keyword(6) | Electrokinetic Forces |
| 1st Author's Name | Moeto NAGAI |
| 1st Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology() |
| 2nd Author's Name | Yuya MATSUSE |
| 2nd Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology |
| 3rd Author's Name | Satoshi SOGA |
| 3rd Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology |
| 4th Author's Name | Sho KAWAHARADA |
| 4th Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology |
| 5th Author's Name | Takahiro KAWASHIMA |
| 5th Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology |
| 6th Author's Name | Takayuki SHIBATA |
| 6th Author's Affiliation | Department of Mechanical Engineering, Toyohashi University of Technology |
| Date | 2014-07-11 |
| Paper # | ED2014-51 |
| Volume (vol) | vol.114 |
| Number (no) | 133 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |