| Presentation | 2014-01-25 Multiplex detection of DNA and RNA viruses using RT-LAMPand metal coated hollow fiber membrane Ryo OI, Toshitsugu SUGAWARA, Satoshi KURODA, Junji ARISAWA, Kazuyuki KIMURA, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We proposed a gene amplification method that enables the detection against small amount of viruses. It is intended for application to nucleic acid amplification test (NAT) that is performed on a subject plasma samples for the Japanese Red Cross Society to ensure the safety of the blood supply. A metal coated hollow fiber membrane was used for detection of virus. The membrane was able to isolate nucleic acids after concentration of virus. Isolated nucleic acids were applied to LAMP (Loop-Mediated Isothermal Amplification). This method was rapid and sensitive detection. In this report, to detect DNA and RNA viruses in one step, we tried to apply nucleic acids of DNA or RNA virus in one step. RT-LAMP could also detect DNA or RNA virus in one step. As a result, be detectable both DNA and RNA viruses also stable were found. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | blood supply / metal-coated hollow fiber membrane / RT-LAMP / Multiplex detection |
| Paper # | MBE2013-110 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2014/1/17(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Multiplex detection of DNA and RNA viruses using RT-LAMPand metal coated hollow fiber membrane |
| Sub Title (in English) | |
| Keyword(1) | blood supply |
| Keyword(2) | metal-coated hollow fiber membrane |
| Keyword(3) | RT-LAMP |
| Keyword(4) | Multiplex detection |
| 1st Author's Name | Ryo OI |
| 1st Author's Affiliation | Graduate School of Engineering, Hokkaido Institute of Technology() |
| 2nd Author's Name | Toshitsugu SUGAWARA |
| 2nd Author's Affiliation | Faculty of medical Engineering, Hokkaido Institute of Technology |
| 3rd Author's Name | Satoshi KURODA |
| 3rd Author's Affiliation | Faculty of medical Engineering, Hokkaido Institute of Technology |
| 4th Author's Name | Junji ARISAWA |
| 4th Author's Affiliation | Faculty of medical Engineering, Hokkaido Institute of Technology |
| 5th Author's Name | Kazuyuki KIMURA |
| 5th Author's Affiliation | Faculty of medical Engineering, Hokkaido Institute of Technology |
| Date | 2014-01-25 |
| Paper # | MBE2013-110 |
| Volume (vol) | vol.113 |
| Number (no) | 409 |
| Page | pp.pp.- |
| #Pages | 5 |
| Date of Issue | |