| Presentation | 2014-01-23 A highly Sensitive Liquid-Phase Detection of Biological Targets with Magnetic Markers and High T_c SQUID Shinobu UCHIDA, Yuta UEOKA, Masakazu URA, Takashi YOSHIDA, Keiji ENPUKU, |
|---|---|
| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We demonstrated a highly sensitive liquid-phase detection of biological targets using magnetic markers and HTS SQUID. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 μΦ_0/Hz^<1/2> in the white noise region. The 1/f noise was 14 μΦ_0/Hz^<1/2> at f=1 Hz when the SQUID was operated with AC bias mode. Using the SQUD system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter 3.3 μm. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 2 × 10^4 corresponding to a sensitivity of 9.5 × 10^<-19> mol/ml. This result indicates a high sensitivity of the developed method. We also show the effect of the excitation field on the signals from the free and bound markers for quantitative evaluation of immunoassay. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Biosensor / Brownian Relaxation / Immunoassay / Magnetic Marker / HTS SQUID |
| Paper # | SCE2013-42 |
| Date of Issue |
| Conference Information | |
| Committee | SCE |
|---|---|
| Conference Date | 2014/1/16(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | |
| Vice Chair | |
| Secretary | |
| Assistant | |
| Paper Information | |
| Registration To | Superconductive Electronics (SCE) |
|---|---|
| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | A highly Sensitive Liquid-Phase Detection of Biological Targets with Magnetic Markers and High T_c SQUID |
| Sub Title (in English) | |
| Keyword(1) | Biosensor |
| Keyword(2) | Brownian Relaxation |
| Keyword(3) | Immunoassay |
| Keyword(4) | Magnetic Marker |
| Keyword(5) | HTS SQUID |
| 1st Author's Name | Shinobu UCHIDA |
| 1st Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University() |
| 2nd Author's Name | Yuta UEOKA |
| 2nd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 3rd Author's Name | Masakazu URA |
| 3rd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 4th Author's Name | Takashi YOSHIDA |
| 4th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University:Research Institute of Superconductor Science and Systems, Kyushu University |
| 5th Author's Name | Keiji ENPUKU |
| 5th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University:Research Institute of Superconductor Science and Systems, Kyushu University |
| Date | 2014-01-23 |
| Paper # | SCE2013-42 |
| Volume (vol) | vol.113 |
| Number (no) | 401 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |