Presentation 2013-04-26
Investigation into directional electron transfer of cytochrome c_3 by electrochemical measurement
Noriyuki Asakura, Masaki DAGI, Mami Fukai, Naoya SHIBUYA,
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Abstract(in English) Cytochrome c_3 is a redox protein containing four hemes in one molecule and works as an electron mediator in vivo. There is intense interest in experiments which reveal, at molecular level, how electrons go into and out of cytochrome c_3. Here we report that specific heme which roles in an entrance or an exit during electron transfer, that is direction of electron transfer through cytochrome c_3, is clarified by electrochemical quartz crystal microbalance (EQCM) measurement. In vivo, cytochrome c_3 accepts/donates electrons from/to hydrogenase, which is catalyze proton reduction and hydrogen oxidation. In this study, electron transfer between cytochrome c_3 and hydrogenase was measured electrochemically, and the electron transfer complex formation was investigated by frequency change of the quartz crystal at the same time as electrochemistry.
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Keyword(in English) cytochrome / electron transfer / redox protein / EQCM
Paper # SDM2013-18,OME2013-18
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Committee OME
Conference Date 2013/4/18(1days)
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Registration To Organic Material Electronics (OME)
Language JPN
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Title (in English) Investigation into directional electron transfer of cytochrome c_3 by electrochemical measurement
Sub Title (in English)
Keyword(1) cytochrome
Keyword(2) electron transfer
Keyword(3) redox protein
Keyword(4) EQCM
1st Author's Name Noriyuki Asakura
1st Author's Affiliation Department of Bioengineering, Tokyo Institute of Technology()
2nd Author's Name Masaki DAGI
2nd Author's Affiliation Department of Bioengineering, Tokyo Institute of Technology
3rd Author's Name Mami Fukai
3rd Author's Affiliation Department of Bioengineering, Tokyo Institute of Technology
4th Author's Name Naoya SHIBUYA
4th Author's Affiliation Department of Bioengineering, Tokyo Institute of Technology
Date 2013-04-26
Paper # SDM2013-18,OME2013-18
Volume (vol) vol.113
Number (no) 18
Page pp.pp.-
#Pages 3
Date of Issue