| Presentation | 2013-04-26 Application of rapid particle manipulation based on dielectrophoresis to bio-analytical systems Tomoyuki YASUKAWA, Fumio MIZUTANI, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have applied particle manipulation based on negative-dielectrophoresis (n-DEP) to develop rapid and separation-free immunosensing systems. A suspension of the fluorescence particles modified with a specific antibody was injected into the n-DEP device consisting of the interdigitated microarray (IDA) electrode and indium-tin-oxide (ITO) substrate immobilized with antibody. The application of AC voltage to the IDA forced the particles to accumulate to the ITO substrate within 60 s. In the presence of analytes, particles were irreversibly captured on the ITO by the construction of immuno-complexes. Particles were re-dispersed after turning off the voltage. We could discriminatively detect the fluorescence intensity of the captured particles from that of the uncaptured particles suspended in the solution. Thus, the separation steps usually required for conventional immunoassay are eliminated in the present procedure. The control of the initial concentration of particles allows to the regulation of the detectable concentration range for the assay. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Particle manipulation / Patterning / Immunosensing / High-sensitivity |
| Paper # | SDM2013-14,OME2013-14 |
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| Conference Information | |
| Committee | OME |
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| Conference Date | 2013/4/18(1days) |
| Place (in Japanese) | (See Japanese page) |
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| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Organic Material Electronics (OME) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Application of rapid particle manipulation based on dielectrophoresis to bio-analytical systems |
| Sub Title (in English) | |
| Keyword(1) | Particle manipulation |
| Keyword(2) | Patterning |
| Keyword(3) | Immunosensing |
| Keyword(4) | High-sensitivity |
| 1st Author's Name | Tomoyuki YASUKAWA |
| 1st Author's Affiliation | Graduate School of Material Science, University of Hyogo:JST-CREST() |
| 2nd Author's Name | Fumio MIZUTANI |
| 2nd Author's Affiliation | Graduate School of Material Science, University of Hyogo |
| Date | 2013-04-26 |
| Paper # | SDM2013-14,OME2013-14 |
| Volume (vol) | vol.113 |
| Number (no) | 18 |
| Page | pp.pp.- |
| #Pages | 5 |
| Date of Issue | |