| Presentation | 2011-01-24 Method of Magnetic-Field Application for Liquid Phase Immunoassay Using SQUID Shunichi CHOSOKABE, Hideki WATANABE, Takashi YOSHIDA, Masaaki MATSUO, Keiji ENPUKU, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have developed a liquid-phase magnetic immunoassay using SQUID. In this method, the antibodies, which specifically bind with antigen, were labeled with magnetic marker. In order to generate the signal from magnetic markers, it is necessary to apply strong external field. However, it causes the aggregation of magnetic markers, which results in the noise of the free markers. In order to solve this problem, we investigated a new method for magnetic field application. In the new method, a strong field was applied for a short time for excitation of markers, while a weaker field was applied during the measurement. It was shown that this two-step application of the external field prevents aggregation of magnetic markers and effectively reduces the noise from the free markers. We demonstrated the detection of 4.7～11.9 femto-mol/l of biotin with this method. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | SQUID / Magnetic marker / Nano Particle / Immunoassay |
| Paper # | SCE2010-44 |
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| Conference Information | |
| Committee | SCE |
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| Conference Date | 2011/1/17(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Vice Chair | |
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| Paper Information | |
| Registration To | Superconductive Electronics (SCE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Method of Magnetic-Field Application for Liquid Phase Immunoassay Using SQUID |
| Sub Title (in English) | |
| Keyword(1) | SQUID |
| Keyword(2) | Magnetic marker |
| Keyword(3) | Nano Particle |
| Keyword(4) | Immunoassay |
| 1st Author's Name | Shunichi CHOSOKABE |
| 1st Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University() |
| 2nd Author's Name | Hideki WATANABE |
| 2nd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 3rd Author's Name | Takashi YOSHIDA |
| 3rd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University:Research Institute of Superconductor Science and Systems, Kyushu University |
| 4th Author's Name | Masaaki MATSUO |
| 4th Author's Affiliation | Research Institute of Superconductor Science and Systems, Kyushu University |
| 5th Author's Name | Keiji ENPUKU |
| 5th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University:Research Institute of Superconductor Science and Systems, Kyushu University |
| Date | 2011-01-24 |
| Paper # | SCE2010-44 |
| Volume (vol) | vol.110 |
| Number (no) | 385 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |