| Presentation | 2010-01-20 Liquid Phase Immunoassay with Susceptibility Measurement of Magnetic Markers Yuuya TAMAI, Tetsu MITAKE, Yu SHAKANO, Masaaki MATSUO, Takashi YOSHIDA, Keiji ENPUKU, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Liquid phase immunoassay using Brownian relaxation of magnetic markers is shown. In this method, difference in the Brownian relaxation time between bound and free markers is used, which can be measured as the difference in the ac susceptibility between them. First, we show the measurement system for the immunoassay. Optimum value of an excitation field, which depends on the magnetic properties of the marker, was set to be 2.5 mT in the present experiment. In this case, we could detect 70 ng of the magnetic markers, which corresponds to the number of 1.6×10^7. Next, we detected biotins that were fixed on the surface of polymer beads. Magnetic markers that were coated with avidin were bound to the polymer beads through the binding reaction between avidin and biotin. Minimum detectable number of the polymer beads was 3.800 for 60 μl sample. Since 3,000 biotins were fixed on the single bead, this result corresponds to the detection of biotins with concentration as small as 0.28 fmol/ml |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | liquid-phase immunoassay / magnetic marker / BoundlFree separation / Brownian relaxation |
| Paper # | SCE2009-30 |
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| Conference Information | |
| Committee | SCE |
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| Conference Date | 2010/1/13(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Superconductive Electronics (SCE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Liquid Phase Immunoassay with Susceptibility Measurement of Magnetic Markers |
| Sub Title (in English) | |
| Keyword(1) | liquid-phase immunoassay |
| Keyword(2) | magnetic marker |
| Keyword(3) | BoundlFree separation |
| Keyword(4) | Brownian relaxation |
| 1st Author's Name | Yuuya TAMAI |
| 1st Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University() |
| 2nd Author's Name | Tetsu MITAKE |
| 2nd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 3rd Author's Name | Yu SHAKANO |
| 3rd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 4th Author's Name | Masaaki MATSUO |
| 4th Author's Affiliation | Research Institute of Superconductor Science and Systems, Kyushu University |
| 5th Author's Name | Takashi YOSHIDA |
| 5th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 6th Author's Name | Keiji ENPUKU |
| 6th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University:Research Institute of Superconductor Science and Systems, Kyushu University |
| Date | 2010-01-20 |
| Paper # | SCE2009-30 |
| Volume (vol) | vol.109 |
| Number (no) | 364 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |