Presentation | 2010-01-29 Label-free imaging of biological samples by stimulated Raman scattering microscopy Yasuyuki OSEKI, Kazuyoshi ITOH, |
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Abstract(in Japanese) | (See Japanese page) |
Abstract(in English) | We have recently developed a technique of label-free imaging of biological samples using stimulated Raman scattering (SRS). This technique utilizes two-color laser pulses and high-frequency lock-in detection to visualize molecular vibrations at a specific frequency. Compared to previous Raman microscopy, SRS microscopy is advantageous in that images can be obtained with high sensitivity and high contrast, and can be analyzed quantitatively. In this paper, we present the principle and imaging results of SRS microscopy, and discuss the advantages of SRS. |
Keyword(in Japanese) | (See Japanese page) |
Keyword(in English) | Stimulated Raman scattering / bioimaging / optical microscope |
Paper # | PN2009-66,OPE2009-204,LQE2009-186 |
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Committee | PN |
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Conference Date | 2010/1/21(1days) |
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Registration To | Photonic Network (PN) |
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Language | JPN |
Title (in Japanese) | (See Japanese page) |
Sub Title (in Japanese) | (See Japanese page) |
Title (in English) | Label-free imaging of biological samples by stimulated Raman scattering microscopy |
Sub Title (in English) | |
Keyword(1) | Stimulated Raman scattering |
Keyword(2) | bioimaging |
Keyword(3) | optical microscope |
1st Author's Name | Yasuyuki OSEKI |
1st Author's Affiliation | Department of Advanced Science and Biotechnology, Osaka Univ.() |
2nd Author's Name | Kazuyoshi ITOH |
2nd Author's Affiliation | Department of Advanced Science and Biotechnology, Osaka Univ. |
Date | 2010-01-29 |
Paper # | PN2009-66,OPE2009-204,LQE2009-186 |
Volume (vol) | vol.109 |
Number (no) | 401 |
Page | pp.pp.- |
#Pages | 6 |
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