Presentation 2009-07-30
Highly sensitive detection of 2,4,6-trinitrotoluene using surface plasmon resonance sensor with displacement immunoassay
Takeshi ONODERA, Yutaka Mizuta, Kazuhiko HORIKAWA, Kiyoshi MATSUMOTO, Norio MIURA, Kiyoshi TOKO,
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Abstract(in English) Trained dogs, metal detectors and X-ray inspection apparatus are mainly used to detect explosives. However, these methods are sometimes difficult to apply for a situation, because they have some disadvantage. In this study, we used antigen-antibody interaction and surface plasmon resonance (SPR) sensor to detect 2,4,6-trinitrotoluene (TNT) molecules with displacement immunoassay and investigated which hapten provided higher sensitivity. As a result, 0.9ppb of detection limit was achieved using DNP-glycine immobilized Au sensor surface via self-assembled monolayers containing ethylene glycol units.
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Keyword(in English) surface plasmon resonance / explosives / trinitrotoluene / antigen-antibody interaction / self-assembled monolayers / displacement / biosensor
Paper # ED2009-106
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Committee ED
Conference Date 2009/7/23(1days)
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Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Highly sensitive detection of 2,4,6-trinitrotoluene using surface plasmon resonance sensor with displacement immunoassay
Sub Title (in English)
Keyword(1) surface plasmon resonance
Keyword(2) explosives
Keyword(3) trinitrotoluene
Keyword(4) antigen-antibody interaction
Keyword(5) self-assembled monolayers
Keyword(6) displacement
Keyword(7) biosensor
1st Author's Name Takeshi ONODERA
1st Author's Affiliation Faculty of Information Science and Electrical Engineering, Kyushu University()
2nd Author's Name Yutaka Mizuta
2nd Author's Affiliation Graduate school of Information Science and Electrical Engineering, Kyushu University
3rd Author's Name Kazuhiko HORIKAWA
3rd Author's Affiliation Graduate school of Information Science and Electrical Engineering, Kyushu University
4th Author's Name Kiyoshi MATSUMOTO
4th Author's Affiliation Faculty of Agriculture, Kyushu University
5th Author's Name Norio MIURA
5th Author's Affiliation Art, Science and Technology Center for Cooperative Research, Kyushu University
6th Author's Name Kiyoshi TOKO
6th Author's Affiliation Faculty of Information Science and Electrical Engineering, Kyushu University
Date 2009-07-30
Paper # ED2009-106
Volume (vol) vol.109
Number (no) 157
Page pp.pp.-
#Pages 6
Date of Issue