| Presentation | 2009-01-24 Study on the antimicrobial activity of Hinokitiol examined by colony counting method and intracellular ATP dose assay Xipeng Zhao, Toshitsugu Sugawara, Satoshi Kuroda, Junji Arisawa, Kazuyuki Kimura, |
|---|---|
| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | In the previous study, we demonstrated that the small amounts of Hinokitiol which isolated from tree oil inhibited bacterial growth effectively. About 50μg/ml (=ppm) of Hinokitiol clearly suppressed either of Escherichia coli and Staphylococcus aureus growth when they were incubated in optimum growth condition like as broth medium, whereas about 100μg/ml (=ppm) of Hinokitiol administration showed bactericidal activity against employed bacteria in same growth condition. In this present study, we conducted the measurement of bacterial metabolic activity using intracellular ATP assay. About 50μg/ml (=ppm) of Hinokitiol did not affect the growth rate of E. coli and Sta. aureus when its were measured by colony forming examination, however, the ATP doses in both of bacterial strains were remarkably decreased by HK administration. This phenomenon indicated that small amount of HK cloud promote the VNC (Viable but not cultured) condition. These result suggested a newly possibility of HK utilization for infection control substance. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Hinokitiol / ATP dose assay |
| Paper # | MBE2008-88 |
| Date of Issue |
| Conference Information | |
| Committee | MBE |
|---|---|
| Conference Date | 2009/1/16(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | |
| Vice Chair | |
| Secretary | |
| Assistant | |
| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
|---|---|
| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Study on the antimicrobial activity of Hinokitiol examined by colony counting method and intracellular ATP dose assay |
| Sub Title (in English) | |
| Keyword(1) | Hinokitiol |
| Keyword(2) | ATP dose assay |
| 1st Author's Name | Xipeng Zhao |
| 1st Author's Affiliation | Division of Applied Electronics, Graduate School of Hokkaido Institute of Technology() |
| 2nd Author's Name | Toshitsugu Sugawara |
| 2nd Author's Affiliation | Department of clinical and Rehabilitation Engineering, Hokkaido Institute of Technology |
| 3rd Author's Name | Satoshi Kuroda |
| 3rd Author's Affiliation | Department of clinical and Rehabilitation Engineering, Hokkaido Institute of Technology |
| 4th Author's Name | Junji Arisawa |
| 4th Author's Affiliation | Department of clinical and Rehabilitation Engineering, Hokkaido Institute of Technology |
| 5th Author's Name | Kazuyuki Kimura |
| 5th Author's Affiliation | Department of clinical and Rehabilitation Engineering, Hokkaido Institute of Technology |
| Date | 2009-01-24 |
| Paper # | MBE2008-88 |
| Volume (vol) | vol.108 |
| Number (no) | 405 |
| Page | pp.pp.- |
| #Pages | 5 |
| Date of Issue | |