| Presentation | 2008-09-26 Image analysis of higher-order nuclear architectureformed by a DNA recombinational repair protein RAD51 Akira ASANO, Kazuteru KONO, Satoshi TASHIRO, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | It has been recently shown that the cell nucleus is a highly compartmentalized organelle with chromosome and chromatin domains formed by DNA. Although nuclear proteins also have been speculated to form higher order structure in the compartment between chromatin domains, how the formation of interchromatin compartment is organized is still unclear. We have previously shown that RAD51, a recombinational repair protein, forms nuclear foci, typically during S phase, and accumulates at sites of DNA double-strand breaks. We found that over-expressed RAD51 forms bundle-like structures in the nucleus. Since the structures are formed in the interchromatin compartment, we decided to study the influence of chromatin structure to the organization of interchromatin compartment. For this purpose, we examined the change of the RAD51 bundle-like structure shape after de-condensation of chromatin by the treatment with trichostatin A (TSA), a histone deacetylase inhibitor. The complexity of the GFP-RAD51 bundle-like structures was analyzed quantitatively using digital image processing methods. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | recombinatorial repair protein / chromatin structure / bioimaging |
| Paper # | SIP2008-97,SIS2008-32 |
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| Conference Information | |
| Committee | SIS |
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| Conference Date | 2008/9/18(1days) |
| Place (in Japanese) | (See Japanese page) |
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| Topics (in Japanese) | (See Japanese page) |
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| Registration To | Smart Info-Media Systems (SIS) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Image analysis of higher-order nuclear architectureformed by a DNA recombinational repair protein RAD51 |
| Sub Title (in English) | |
| Keyword(1) | recombinatorial repair protein |
| Keyword(2) | chromatin structure |
| Keyword(3) | bioimaging |
| 1st Author's Name | Akira ASANO |
| 1st Author's Affiliation | Department of Information Engineering, Gradute School of Engineering, Hiroshima University() |
| 2nd Author's Name | Kazuteru KONO |
| 2nd Author's Affiliation | Research Institute for Radiation Biology and Medicine, Hiroshima University |
| 3rd Author's Name | Satoshi TASHIRO |
| 3rd Author's Affiliation | Research Institute for Radiation Biology and Medicine, Hiroshima University |
| Date | 2008-09-26 |
| Paper # | SIP2008-97,SIS2008-32 |
| Volume (vol) | vol.108 |
| Number (no) | 214 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |