| Presentation | 2008-03-13 On classification of cell types in rat substantia nigra pars compacta on the basis of electrophysiological properties of neural membranes Ryohei IWAHASHI, Takashi TATENO, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | In this study, to aim to understand characteristics of local circuits in substantia nigra pars compacta (SNc) of basal ganglia, we carried out electrophysiological experiments for rat midbrain slices at the single cell level. To investigate a possibility to classify neurons in SNc into several cell-types, especially, we recorded membrane voltage and current signals from individual neurons using a patch-clamp recording technique. Firstly, our results showed, as reported in previous studies, that neurons were classified into two cell-types; principal cells (PCs) and secondary cells (SCs). Firing rate in most of the PCs was lower than that in the SCs, and spike width in the PCs was wider than that in the SCs. Secondly, we immunochemically tested if the PCs were dopaminergic (DA) neurons and knew that non-DA neurons were included in the PCs. Thirdly, we tried to find subtypes in the SCs on the basis of obtained electrophysiological data. However, our analysis indicated that it is difficult to classify the SCs into several subtypes, because of continuous cell distribution in a space of some characteristic parameters of firing properties. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | dopaminergic neuron / immunochemical staining / patch clamp recording technique |
| Paper # | NC2007-155 |
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| Committee | NC |
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| Conference Date | 2008/3/5(1days) |
| Place (in Japanese) | (See Japanese page) |
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| Topics (in Japanese) | (See Japanese page) |
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| Registration To | Neurocomputing (NC) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | On classification of cell types in rat substantia nigra pars compacta on the basis of electrophysiological properties of neural membranes |
| Sub Title (in English) | |
| Keyword(1) | dopaminergic neuron |
| Keyword(2) | immunochemical staining |
| Keyword(3) | patch clamp recording technique |
| 1st Author's Name | Ryohei IWAHASHI |
| 1st Author's Affiliation | Graduate School of Engineering Science, Osaka University() |
| 2nd Author's Name | Takashi TATENO |
| 2nd Author's Affiliation | Graduate School of Engineering Science, Osaka University:PRESTO, Japan Science and Technology Agency |
| Date | 2008-03-13 |
| Paper # | NC2007-155 |
| Volume (vol) | vol.107 |
| Number (no) | 542 |
| Page | pp.pp.- |
| #Pages | 6 |
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