Presentation | 2007-11-09 Preparation of an Enzymatic Glucose Sensor based on Hybrid Organic-Inorganic Langmuir-Bodgett Films : Adsorption of Glucose Oxidase into Positively Charged Molecular Layers Hitoshi OHNUKI, Akira KUSAKARI, Mitsuru IZUMI, |
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Abstract(in Japanese) | (See Japanese page) |
Abstract(in English) | In this report, the adsorption mechanism of glucose oxidase (GOx) into positively charged Langmuir-Blodgett (LB) films is described. The glucose oxidase (GOx) was immobilized in the hybrid organic-inorganic Langmuir-Blodgett (LB) films consisting of positively charged octadecyltrimethylammonium (ODTA) and nano-sized Prussian blue (PB) clusters. The ODTA/PB/GOx LB films showed a glucose sensor behavior at a very low operating potential of 0.0V (vs. Ag/AgCl), which character is effective to inhibit the responses from interferents such as ascorbic and uric acids. We investigated the sensor's response current density as a function of the pH of GOx adsorption solution. It was found that the current density significantly depended on the pH. The maximum current density, whose values reached 4 times as large as that of ordinary one, was obtained at pH 4 where is near the isoelectric point of GOx. Infrared absorption spectra revealed that such dependence of the current density was caused by the amount of GOx. A series of models based on the electrostatic attractive force between GOx and ODTA can well explain the observed pH effect on the GOx adsorption. These findings are useful for immobilizing GOx through electrostatic interaction with positively charged molecules. |
Keyword(in Japanese) | (See Japanese page) |
Keyword(in English) | Biosensors / Glucose oxidase / Langmuir-Blodgett Films / Prussian blue |
Paper # | OME2007-55 |
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Committee | OME |
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Conference Date | 2007/11/2(1days) |
Place (in Japanese) | (See Japanese page) |
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Registration To | Organic Material Electronics (OME) |
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Language | JPN |
Title (in Japanese) | (See Japanese page) |
Sub Title (in Japanese) | (See Japanese page) |
Title (in English) | Preparation of an Enzymatic Glucose Sensor based on Hybrid Organic-Inorganic Langmuir-Bodgett Films : Adsorption of Glucose Oxidase into Positively Charged Molecular Layers |
Sub Title (in English) | |
Keyword(1) | Biosensors |
Keyword(2) | Glucose oxidase |
Keyword(3) | Langmuir-Blodgett Films |
Keyword(4) | Prussian blue |
1st Author's Name | Hitoshi OHNUKI |
1st Author's Affiliation | Faculty of Marine Technology, Tokyo University of Marine Science and Technology() |
2nd Author's Name | Akira KUSAKARI |
2nd Author's Affiliation | Faculty of Marine Technology, Tokyo University of Marine Science and Technology |
3rd Author's Name | Mitsuru IZUMI |
3rd Author's Affiliation | Faculty of Marine Technology, Tokyo University of Marine Science and Technology |
Date | 2007-11-09 |
Paper # | OME2007-55 |
Volume (vol) | vol.107 |
Number (no) | 307 |
Page | pp.pp.- |
#Pages | 5 |
Date of Issue |