Presentation 2006-12-18
Fabrication and fluorescence measurement system for microarray using Digital Micromirror Device
Naoya SATO, Yuki HASEGAWA, Hidekazu UCHIDA,
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Abstract(in English) We developed a new system that can totally automate a microarray process from well fabrication until fluorescence measurement using Digital Micromirror Device (DMD). The microarray was fabricated by maskless photolithography of photoresist SU-8 exposed by DMD. This method can fabricate up to 10,000 wells in one inch square area. Fluorescence of the microarray was sequentially measured by excitation of individual well using DMD. Detection of the fluorescence was performed by a photomultiplier tube (PMT). For the system evaluation, we measured a microarray of 25 wells (1μL/well) that were filled up with Lucifer Yellow (a fluorescent material) aqueous solution. As a result, the system with a dichroic mirror to reflect an excitation ray in 90 deg can measure 0.01mg/mL on signal to noise ratio of 7.51.
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Keyword(in English) Microarray / Digital Micromirror Device (DMD) / photolithography / Photomultiplier tube (PMT)
Paper # OME2006-108
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Committee OME
Conference Date 2006/12/11(1days)
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Registration To Organic Material Electronics (OME)
Language ENG
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Fabrication and fluorescence measurement system for microarray using Digital Micromirror Device
Sub Title (in English)
Keyword(1) Microarray
Keyword(2) Digital Micromirror Device (DMD)
Keyword(3) photolithography
Keyword(4) Photomultiplier tube (PMT)
1st Author's Name Naoya SATO
1st Author's Affiliation Graduate School of Science and Engineering, Saitama University()
2nd Author's Name Yuki HASEGAWA
2nd Author's Affiliation Graduate School of Science and Engineering, Saitama University
3rd Author's Name Hidekazu UCHIDA
3rd Author's Affiliation Graduate School of Science and Engineering, Saitama University
Date 2006-12-18
Paper # OME2006-108
Volume (vol) vol.106
Number (no) 439
Page pp.pp.-
#Pages 6
Date of Issue