| Presentation | 2006-07-06 Liquid Phase Immunoassay Utilizing Magnetic Marker and SQUID Takashi NISHIMOTO, Takashi MATSUDA, Hidetoshi TOKUMITSU, Tsuyoshi TANAKA, Keiji ENPUKU, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have developed a liquid phase-immunoassay system utilizing SQUID and magnetic marker. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed to show the remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUID. The measurement was performed in a solution that contains both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers was used to distinguish the bound markers from the free ones. The spurious signal from the free markers, which was caused by imperfectness of the Brownian rotation, was studied. Using the system, we showed the detection of the biological material called IgE without BF separation. At present, we could detect the IgE down to 7pg (or 39 atto-mol). Since the sensitivity was limited by the spurious signal from the free makers, the sensitivity will be improved by improving the dispersion of the markers. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | SQUID / Immunoassay / BF separation / Magnetic marker / Brownian rotation |
| Paper # | SCE2006-19 |
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| Conference Information | |
| Committee | SCE |
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| Conference Date | 2006/6/29(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Superconductive Electronics (SCE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Liquid Phase Immunoassay Utilizing Magnetic Marker and SQUID |
| Sub Title (in English) | |
| Keyword(1) | SQUID |
| Keyword(2) | Immunoassay |
| Keyword(3) | BF separation |
| Keyword(4) | Magnetic marker |
| Keyword(5) | Brownian rotation |
| 1st Author's Name | Takashi NISHIMOTO |
| 1st Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University() |
| 2nd Author's Name | Takashi MATSUDA |
| 2nd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 3rd Author's Name | Hidetoshi TOKUMITSU |
| 3rd Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 4th Author's Name | Tsuyoshi TANAKA |
| 4th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| 5th Author's Name | Keiji ENPUKU |
| 5th Author's Affiliation | Graduate School of Information Science and Electrical Engineering, Kyushu University |
| Date | 2006-07-06 |
| Paper # | SCE2006-19 |
| Volume (vol) | vol.106 |
| Number (no) | 139 |
| Page | pp.pp.- |
| #Pages | 6 |
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