| Presentation | 2006-01-27 Application of the synaptic bouton preparation to rat retinal ganglion cells Tamami MOTOMURA, Yuki HAYASIDA, Norio AKAIKE, Nobuki MURAYAMA, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | The vibrodissociation method has been applied to various CNS regions, yet never applied to vertebrate retinas. This study is aimed to examine whether this method is valid to isolate ganglion cells from a mammalian retina. In perforated-patch whole-cell recordings, the isolated ganglion cells showed sustained as well as transient spiking in response to depolarizing constant current injections, and showed voltage-gated Na^+ current in response to depolarization steps. Under current-clamp, the ganglion cells somata showed spontaneous IPSP-like events of which reversal potential was approximately -80 mV. The present study suggested that the vibrodissociation method is useful to isolate rat retinal ganglion cells attached with inhibitory synaptic boutons. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | retina / ganglion cell / synaptic bouton preparation / electrophysiological experiment |
| Paper # | MBE2005-108 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2006/1/20(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Application of the synaptic bouton preparation to rat retinal ganglion cells |
| Sub Title (in English) | |
| Keyword(1) | retina |
| Keyword(2) | ganglion cell |
| Keyword(3) | synaptic bouton preparation |
| Keyword(4) | electrophysiological experiment |
| 1st Author's Name | Tamami MOTOMURA |
| 1st Author's Affiliation | Faculty of Engineering, Kumamoto University() |
| 2nd Author's Name | Yuki HAYASIDA |
| 2nd Author's Affiliation | Faculty of Engineering, Kumamoto University |
| 3rd Author's Name | Norio AKAIKE |
| 3rd Author's Affiliation | Research Division for Life Science, Kumamoto Health Science University |
| 4th Author's Name | Nobuki MURAYAMA |
| 4th Author's Affiliation | Faculty of Engineering, Kumamoto University |
| Date | 2006-01-27 |
| Paper # | MBE2005-108 |
| Volume (vol) | vol.105 |
| Number (no) | 577 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |