| Presentation | 2005/3/22 Optical Monitoring of Network Activities in Dentate Gyrus of Rat Hippocampal Slices Masanori Murayama, Kenichi Miyazaki, Yoshihisa Kudo, Hiroyoshi Miyakawa, Masashi Inoue, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Monitoring neuronal activities from multiple neurons is essential for understanding neuronal network activities. We have developed a novel method to label multiple granule cells retrogradely with a Ca^<2+> indicator in rat hippocampal slices. Synchronized burst activities, which were induced by applying 4-aminopyridine, were monitored extracellularly with a glass electrode placed at the granule cell layer in the dentate gyrus. During the burst activities, action potential-induced Ca^<2+> transients from multiple (4 - 12) granule cells were monitored simultaneously with a cooled CCD camera with single-cell resolution. Temporal structures of firing patterns from the multiple neurons were determined from Ca^<2+> imaging data. We found that temporal synchrony of the action potentials within the single burst event was enhanced as the burst activities proceeded. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Hippocampal slices / Calcium imaging / Burst activities / Spike synchrony / Granule cell |
| Paper # | NC2004-172 |
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| Conference Information | |
| Committee | NC |
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| Conference Date | 2005/3/22(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Neurocomputing (NC) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Optical Monitoring of Network Activities in Dentate Gyrus of Rat Hippocampal Slices |
| Sub Title (in English) | |
| Keyword(1) | Hippocampal slices |
| Keyword(2) | Calcium imaging |
| Keyword(3) | Burst activities |
| Keyword(4) | Spike synchrony |
| Keyword(5) | Granule cell |
| 1st Author's Name | Masanori Murayama |
| 1st Author's Affiliation | Tokyo University of Pharmacy and Life Science() |
| 2nd Author's Name | Kenichi Miyazaki |
| 2nd Author's Affiliation | Tokyo University of Pharmacy and Life Science |
| 3rd Author's Name | Yoshihisa Kudo |
| 3rd Author's Affiliation | Tokyo University of Pharmacy and Life Science |
| 4th Author's Name | Hiroyoshi Miyakawa |
| 4th Author's Affiliation | Tokyo University of Pharmacy and Life Science |
| 5th Author's Name | Masashi Inoue |
| 5th Author's Affiliation | Tokyo University of Pharmacy and Life Science |
| Date | 2005/3/22 |
| Paper # | NC2004-172 |
| Volume (vol) | vol.104 |
| Number (no) | 759 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |