| Presentation | 2005-06-17 A basic study on sonoporation caused by pulsed ultrasoud with microbubbles : Evaluation of cell membrane damage and repair using scanning electron microscopy and fluorescent microscopy Masayasu IKEBUCHI, Kengo OKADA, Nobuki KUDO, Katsuyuki YAMAMOTO, |
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| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Sonoporation is a technique that increases drug uptake of cells using ultrasound exposure. Recently, it has been shown that exposure of cells to pulsed ultrasound with microbubbles attached to the cells can improve efficiency of sonoporation; however, the mechanisms of this phenomenon have not yet been revealed. In this study, we carried out sonoporation of cultivated endothelial cells using pulsed ultrasound in the presence of bubbles, and the cell slight damage and repair was evaluated using fluorescence and scanning electron microscopes. Based on the results, we concluded that mechanical damage of cell membrane induced by mechanical bubble effects can enhance cell membrane permeability and that damage of cell membrane is repaired within 20 seconds. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | sonoporation / pulsed ultrasound / microbubbles / cell membrane damage and repair |
| Paper # | MBE2005-17 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2005/6/10(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | A basic study on sonoporation caused by pulsed ultrasoud with microbubbles : Evaluation of cell membrane damage and repair using scanning electron microscopy and fluorescent microscopy |
| Sub Title (in English) | |
| Keyword(1) | sonoporation |
| Keyword(2) | pulsed ultrasound |
| Keyword(3) | microbubbles |
| Keyword(4) | cell membrane damage and repair |
| 1st Author's Name | Masayasu IKEBUCHI |
| 1st Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University() |
| 2nd Author's Name | Kengo OKADA |
| 2nd Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University |
| 3rd Author's Name | Nobuki KUDO |
| 3rd Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University |
| 4th Author's Name | Katsuyuki YAMAMOTO |
| 4th Author's Affiliation | Graduate School of Information Science and Technology, Hokkaido University |
| Date | 2005-06-17 |
| Paper # | MBE2005-17 |
| Volume (vol) | vol.105 |
| Number (no) | 122 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |