| Presentation | 2002/3/11 Functional roles of nitric oxide in the regulation of contraction rhythm during simulated ischemia-reperfusion in cultured cardiac myocytes Reina Abe, Masayuki Kohashi, Yoshiko Yamauchi, Koichi Kawahara, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | In this study, we have tried to elucidate the functional significance of nitric oxide (NO) during in vitro ischemia (simulated ischemia) in spontaneously beating cultured cardiac myocytes. Simulated ischemia resulted in the gradual increase of contraction intervals in cardiac myocytes with time. The myocytes finally stopped spontaneous contraction in 2 h from the onset of ischemia. Treatment of cultures either with an inhibitor of nitric oxide synthase (NOS) or with an NO scavenger during ischemia markedly reduced the period from the onset of ischemia to the cessation of contraction compared with that observed in cultures without such treatment. The treatment also resulted in the significant increase in the death of myocytes after reperfusion for 20 h. This study suggested that NO produced during ischemia would play important roles in the regulation of contraction rhythm as well as in the protection from ischemia-induced death of cardiac myocytes. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | nitric oxide / cultured cardiac myocytes / contraction rhythm / ischemia |
| Paper # | MBE2001-178 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2002/3/11(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Functional roles of nitric oxide in the regulation of contraction rhythm during simulated ischemia-reperfusion in cultured cardiac myocytes |
| Sub Title (in English) | |
| Keyword(1) | nitric oxide |
| Keyword(2) | cultured cardiac myocytes |
| Keyword(3) | contraction rhythm |
| Keyword(4) | ischemia |
| 1st Author's Name | Reina Abe |
| 1st Author's Affiliation | Lab.of Biomedical Control,Research Institute for Electronic Science, Hokkaido University() |
| 2nd Author's Name | Masayuki Kohashi |
| 2nd Author's Affiliation | Lab.of Biomedical Control,Research Institute for Electronic Science, Hokkaido University |
| 3rd Author's Name | Yoshiko Yamauchi |
| 3rd Author's Affiliation | Lab.of Biomedical Control,Research Institute for Electronic Science, Hokkaido University |
| 4th Author's Name | Koichi Kawahara |
| 4th Author's Affiliation | Lab.of Biomedical Control,Research Institute for Electronic Science, Hokkaido University |
| Date | 2002/3/11 |
| Paper # | MBE2001-178 |
| Volume (vol) | vol.101 |
| Number (no) | 733 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |