| Presentation | 2000/8/15 OME2000-94 SPR immunosensors for detection of drugs(morphine and methamphetamine) Go Sakai, Taizo Uda, Norio Miura, Noboru Yamazoe, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Highly sensitive and selective detection of drugs(morphine and methamphetamine)was realized by using immunosensor based on surface-plasmon-resonance(SPR)phenomena. A drug-bovine serum albumin(drug-BSA)conjugate was prepared as an antigen. The conjugate was immobilized on the Au thin film of sensor chip by physical adsorption. The incident angle of the drug-BSA immobilized sensor increased with incresing concentration of anti-drug-BSA antibody. The addition of the drugs into the antibody solution was found to decrease the incident angle shift sharply because of the inhibition effect of the drugs. The present sensor was confirmed to detect morphine and methamphetamine very sensitively in the concentration range of 0.1-10ng/ml(ppb)and 0.1-1000ng/ml(ppb), respectively. From the correlation between incident angle shift and drug concentration, the affinity constants of each systems were evaluated by assuming Langmuir isotherm and equilibrium state of immunoreaction. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Immunoreaction / SPR / Drug / Morphine / Methamphetamine / Inhibition |
| Paper # | OME2000-94 |
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| Conference Information | |
| Committee | OME |
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| Conference Date | 2000/8/15(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Organic Material Electronics (OME) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | OME2000-94 SPR immunosensors for detection of drugs(morphine and methamphetamine) |
| Sub Title (in English) | |
| Keyword(1) | Immunoreaction |
| Keyword(2) | SPR |
| Keyword(3) | Drug |
| Keyword(4) | Morphine |
| Keyword(5) | Methamphetamine |
| Keyword(6) | Inhibition |
| 1st Author's Name | Go Sakai |
| 1st Author's Affiliation | Department of Molecular and Material Sciences, Graduate School of Engineering Sciences, Kyushu University() |
| 2nd Author's Name | Taizo Uda |
| 2nd Author's Affiliation | School of Bioresources, Hiroshima Prefectural University |
| 3rd Author's Name | Norio Miura |
| 3rd Author's Affiliation | Advanced Science and Technology Center for Cooperative Research, Kyushu University |
| 4th Author's Name | Noboru Yamazoe |
| 4th Author's Affiliation | Department of Molecular and Material Sciences, Graduate School of Engineering Sciences, Kyushu University |
| Date | 2000/8/15 |
| Paper # | OME2000-94 |
| Volume (vol) | vol.100 |
| Number (no) | 253 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |