| Presentation | 1999/6/25 Development of DNA sensor for the detection of Escherichia coli O157 Kazunori Ikebukuro, Eriko Kai, Kazuyoshi Yano, Isao Karube, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Polymerase chain reaction (PCR) products were detected using a flow injection type sensor based on surface plasmon resonance (SPR). Asymmetric PCR was used to amplify the target DNA sequence and two products with different length were produced. The novelty of our DNA detection system was that our target DNA was double stranded but the probe binding site, located in the 3-terminus, was single stranded. This avoids the formation of intra-and intermolecular complexes. This novel design permitted us not only to detect PCR product with high-sensitivity, but also to develop a rapid detection system for the detection of the verotoxin 2 gene of Escherichia coli O157:H7. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Escherichia coli O157:H7 / DNA detection / verotoxin / Surface plasmon resonance |
| Paper # | OME99-34 |
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| Conference Information | |
| Committee | OME |
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| Conference Date | 1999/6/25(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
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| Vice Chair | |
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| Paper Information | |
| Registration To | Organic Material Electronics (OME) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Development of DNA sensor for the detection of Escherichia coli O157 |
| Sub Title (in English) | |
| Keyword(1) | Escherichia coli O157:H7 |
| Keyword(2) | DNA detection |
| Keyword(3) | verotoxin |
| Keyword(4) | Surface plasmon resonance |
| 1st Author's Name | Kazunori Ikebukuro |
| 1st Author's Affiliation | Research Center for Advanced Science and Technology, University of Tokyo() |
| 2nd Author's Name | Eriko Kai |
| 2nd Author's Affiliation | Research Center for Advanced Science and Technology, University of Tokyo |
| 3rd Author's Name | Kazuyoshi Yano |
| 3rd Author's Affiliation | Research Center for Advanced Science and Technology, University of Tokyo |
| 4th Author's Name | Isao Karube |
| 4th Author's Affiliation | Research Center for Advanced Science and Technology, University of Tokyo |
| Date | 1999/6/25 |
| Paper # | OME99-34 |
| Volume (vol) | vol.99 |
| Number (no) | 159 |
| Page | pp.pp.- |
| #Pages | 5 |
| Date of Issue | |