Presentation | 1994/1/13 Fluorescence measurement of membrane potential using organic dye Kunihiko Maruyama, Yasumitsu Miyazaki, |
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Abstract(in Japanese) | (See Japanese page) |
Abstract(in English) | One of the most popular optical mode for monitoring membrane potential is fluorescence.The purpose of this research is to develop a micro optical probe system for measuring membrane potential,in which fluorescent dye:NK2612 is used.We confirmed The beamwaist of incident laser light is 1.5μm,the absorption maximum is 767nm and the fluorescent maximum is 843 nm.Furthermore,we optimized the concentration of the fluorescent dye,confirmed fluorescence intensity is maximum when the concentration of the dye is 12.5μg, ml.We observed the dependence of fluorescence inten sity on the membrane potential and showed micro optical probe system is available for measuring membrane potential by fluorescence. |
Keyword(in Japanese) | (See Japanese page) |
Keyword(in English) | Ion channel / Fluorescent dye / Membrane potential / Micro optical probe system |
Paper # | OME93-48,DEI-94-3 |
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Conference Information | |
Committee | OME |
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Conference Date | 1994/1/13(1days) |
Place (in Japanese) | (See Japanese page) |
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Paper Information | |
Registration To | Organic Material Electronics (OME) |
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Language | JPN |
Title (in Japanese) | (See Japanese page) |
Sub Title (in Japanese) | (See Japanese page) |
Title (in English) | Fluorescence measurement of membrane potential using organic dye |
Sub Title (in English) | |
Keyword(1) | Ion channel |
Keyword(2) | Fluorescent dye |
Keyword(3) | Membrane potential |
Keyword(4) | Micro optical probe system |
1st Author's Name | Kunihiko Maruyama |
1st Author's Affiliation | Toyohashi University of Technology() |
2nd Author's Name | Yasumitsu Miyazaki |
2nd Author's Affiliation | Toyohashi University of Technology |
Date | 1994/1/13 |
Paper # | OME93-48,DEI-94-3 |
Volume (vol) | vol.93 |
Number (no) | 406 |
Page | pp.pp.- |
#Pages | 6 |
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