Presentation 1994/1/13
Fluorescence measurement of membrane potential using organic dye
Kunihiko Maruyama, Yasumitsu Miyazaki,
PDF Download Page PDF download Page Link
Abstract(in Japanese) (See Japanese page)
Abstract(in English) One of the most popular optical mode for monitoring membrane potential is fluorescence.The purpose of this research is to develop a micro optical probe system for measuring membrane potential,in which fluorescent dye:NK2612 is used.We confirmed The beamwaist of incident laser light is 1.5μm,the absorption maximum is 767nm and the fluorescent maximum is 843 nm.Furthermore,we optimized the concentration of the fluorescent dye,confirmed fluorescence intensity is maximum when the concentration of the dye is 12.5μg, ml.We observed the dependence of fluorescence inten sity on the membrane potential and showed micro optical probe system is available for measuring membrane potential by fluorescence.
Keyword(in Japanese) (See Japanese page)
Keyword(in English) Ion channel / Fluorescent dye / Membrane potential / Micro optical probe system
Paper # OME93-48,DEI-94-3
Date of Issue

Conference Information
Committee OME
Conference Date 1994/1/13(1days)
Place (in Japanese) (See Japanese page)
Place (in English)
Topics (in Japanese) (See Japanese page)
Topics (in English)
Chair
Vice Chair
Secretary
Assistant

Paper Information
Registration To Organic Material Electronics (OME)
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Fluorescence measurement of membrane potential using organic dye
Sub Title (in English)
Keyword(1) Ion channel
Keyword(2) Fluorescent dye
Keyword(3) Membrane potential
Keyword(4) Micro optical probe system
1st Author's Name Kunihiko Maruyama
1st Author's Affiliation Toyohashi University of Technology()
2nd Author's Name Yasumitsu Miyazaki
2nd Author's Affiliation Toyohashi University of Technology
Date 1994/1/13
Paper # OME93-48,DEI-94-3
Volume (vol) vol.93
Number (no) 406
Page pp.pp.-
#Pages 6
Date of Issue