Presentation 1997/10/9
Develpoment of high-sensitive DNA sensor
Koji Hashimoto,
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Abstract(in Japanese) (See Japanese page)
Abstract(in English) An electrochemical DNA detection method (DNA sensor) has been developing for the measurement of hepatitis B virus (HBV) in human sera. The DNA sensor uses a three-step process to determine the concentration of HBV. First, oligonucleotides (DNA probes) immobilized on the gold electrode of the DNA sensor selectively catch HBV DNA. Second, DNA minor groove binder (Hoechst 33258) acting as and electrochemical signal amplifier reacts with the DNA hybrids formed on the electrode. Third, the current signal from the Hoechst 33258 is measured. A correlation coefficient of 0.75 was obtained between the 52 patients' data from the DNA sensor and those from the competitive polymerase chain reaction (PCR) method (10^3-10^8 copies/40μL).
Keyword(in Japanese) (See Japanese page)
Keyword(in English) DNA sensor / electrochemical / Hoechst 33258 / Hepatitis B virus (HBV)
Paper # OME97-78-81
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Conference Information
Committee OME
Conference Date 1997/10/9(1days)
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Place (in English)
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Paper Information
Registration To Organic Material Electronics (OME)
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Develpoment of high-sensitive DNA sensor
Sub Title (in English)
Keyword(1) DNA sensor
Keyword(2) electrochemical
Keyword(3) Hoechst 33258
Keyword(4) Hepatitis B virus (HBV)
1st Author's Name Koji Hashimoto
1st Author's Affiliation Environmental Engineering Department, Research and Development Center, Toshiba Corporation()
Date 1997/10/9
Paper # OME97-78-81
Volume (vol) vol.97
Number (no) 308
Page pp.pp.-
#Pages 4
Date of Issue