| Presentation | 1997/10/9 Develpoment of high-sensitive DNA sensor Koji Hashimoto, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | An electrochemical DNA detection method (DNA sensor) has been developing for the measurement of hepatitis B virus (HBV) in human sera. The DNA sensor uses a three-step process to determine the concentration of HBV. First, oligonucleotides (DNA probes) immobilized on the gold electrode of the DNA sensor selectively catch HBV DNA. Second, DNA minor groove binder (Hoechst 33258) acting as and electrochemical signal amplifier reacts with the DNA hybrids formed on the electrode. Third, the current signal from the Hoechst 33258 is measured. A correlation coefficient of 0.75 was obtained between the 52 patients' data from the DNA sensor and those from the competitive polymerase chain reaction (PCR) method (10^3-10^8 copies/40μL). |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | DNA sensor / electrochemical / Hoechst 33258 / Hepatitis B virus (HBV) |
| Paper # | OME97-78-81 |
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| Conference Information | |
| Committee | OME |
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| Conference Date | 1997/10/9(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | |
| Vice Chair | |
| Secretary | |
| Assistant | |
| Paper Information | |
| Registration To | Organic Material Electronics (OME) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Develpoment of high-sensitive DNA sensor |
| Sub Title (in English) | |
| Keyword(1) | DNA sensor |
| Keyword(2) | electrochemical |
| Keyword(3) | Hoechst 33258 |
| Keyword(4) | Hepatitis B virus (HBV) |
| 1st Author's Name | Koji Hashimoto |
| 1st Author's Affiliation | Environmental Engineering Department, Research and Development Center, Toshiba Corporation() |
| Date | 1997/10/9 |
| Paper # | OME97-78-81 |
| Volume (vol) | vol.97 |
| Number (no) | 308 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |