Presentation | 1997/10/9 Fabrication and Function Characterization of Protein Crystalline Films Kuniaki Nagayama, |
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Abstract(in Japanese) | (See Japanese page) |
Abstract(in English) | Functional films made of protein molecules are now receiving much attention by its prospect of application to biosensors, bioelectronics and molecular sieves. As an effcient way of making functional films, we have developed a method using very thin liquid films in which protein molecules are two-dimensionally confined and arrayed. With the liquid film method, two-dimensional crystalline films have teen made of an iron-storege protein, ferritin. Crystalline films have a virtue that all the molecules inside are oriented in one and the same direction. To obtain a film consisted of function-preserved protein molecules with a desired orientation, we employed a genetic engineering approach to control the protein-protein interaction. To test the function preservation of ferritin in the crystalline film state adsorped on a solid substrate, we have developed an electron microscope method to observe the iron-incorporation activity of indivisual ferritin molecules. The functional preservation was confirmed with varying figures of 60 to 85% to the total amount of adsorped ferritin molecules. |
Keyword(in Japanese) | (See Japanese page) |
Keyword(in English) | proteins / crystalline film / function film / ferritin / two-dimensional crystal / function characterization |
Paper # | OME97-78-81 |
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Conference Information | |
Committee | OME |
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Conference Date | 1997/10/9(1days) |
Place (in Japanese) | (See Japanese page) |
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Topics (in Japanese) | (See Japanese page) |
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Paper Information | |
Registration To | Organic Material Electronics (OME) |
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Language | JPN |
Title (in Japanese) | (See Japanese page) |
Sub Title (in Japanese) | (See Japanese page) |
Title (in English) | Fabrication and Function Characterization of Protein Crystalline Films |
Sub Title (in English) | |
Keyword(1) | proteins |
Keyword(2) | crystalline film |
Keyword(3) | function film |
Keyword(4) | ferritin |
Keyword(5) | two-dimensional crystal |
Keyword(6) | function characterization |
1st Author's Name | Kuniaki Nagayama |
1st Author's Affiliation | National Institute for Physiological Sciences() |
Date | 1997/10/9 |
Paper # | OME97-78-81 |
Volume (vol) | vol.97 |
Number (no) | 308 |
Page | pp.pp.- |
#Pages | 6 |
Date of Issue |