| Presentation | 1997/6/27 Screening and application of enzymes suitable for biosensor component Wakako Tsugawa, Koji Sode, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | The screening for bacterial enzymes for the biosensor has been carried outt. We isolated PQQ glucose dehydrognase from marine bacteria, strain N11. It has NaCl dependent activity, which was unique for marine bacterial enzyme. Two glucose dehydrogenases which could oxidize C-3 hydroxy group of glucose were found in marine bacteria. One was isolated from membrane fraction of Cytophaga marinoflava and the other was from soluble fraction of α-15strain. Using these enzymes, we developped an enzyme sensor and a novel fluorescent measurement method for 1,5-anhydroglucitol, diabetes marker in human serum. Strain SM4 was isolated from a soil near the hot spring. Its GDH showed unique temperature profile and thermal stability. Microbial sensor was constructed by using 1,5-anhydroglucitol assimilating bacterium. PQQGDH-overexpression changed substrate specificity of E. coli's respiratory activity. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | biosensor / glucose dehydrogenase / marine bacteria / 1,5-anhydroglucitol / heat stable enzyme / respiration |
| Paper # | CPM97-31 |
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| Conference Information | |
| Committee | OME |
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| Conference Date | 1997/6/27(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | |
| Vice Chair | |
| Secretary | |
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| Paper Information | |
| Registration To | Organic Material Electronics (OME) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Screening and application of enzymes suitable for biosensor component |
| Sub Title (in English) | |
| Keyword(1) | biosensor |
| Keyword(2) | glucose dehydrogenase |
| Keyword(3) | marine bacteria |
| Keyword(4) | 1,5-anhydroglucitol |
| Keyword(5) | heat stable enzyme |
| Keyword(6) | respiration |
| 1st Author's Name | Wakako Tsugawa |
| 1st Author's Affiliation | Tokyo University of Agriculture and Technology Department of biotechnology() |
| 2nd Author's Name | Koji Sode |
| 2nd Author's Affiliation | Tokyo University of Agriculture and Technology Department of biotechnology |
| Date | 1997/6/27 |
| Paper # | CPM97-31 |
| Volume (vol) | vol.97 |
| Number (no) | 144 |
| Page | pp.pp.- |
| #Pages | 4 |
| Date of Issue | |