Presentation 1996/6/21
Fabrication of Optical Biosensor Based on LB Films of Biosynthetically Lipid-Tagged Antibody
Yoshiki Hirata, Marja-Leena Laukkanen, Kari Keinanen, Etsuo Shinohara, Fumio Mizutani, Tasuo Katsura, Masuo Aizawa,
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Abstract(in English) The immobilization of antibody molecules is a key step in fabrication of immunosensors. Recently, an anti-2-phenyloxazollone lipid-tagged single chain antibody was biosynthetically produced by fusion with the signal peptide of the major lipoprotein of E. coli. We have investigated the immobilization of such antibody using the Langmuir Blodgett (LB) technique because it can control molecular orientation in monolayer level. Orientation and distribution of antibody in monolayer and kinetics of antigen binding are studied by using florescence microscopy and surface plasmon resonance (SPR). A stable mixed monolayer was formed by direct spreading of liposomes containing this fusion antibody. The transferred monolayer shows antigen binding activity on SPR response.
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Keyword(in English) antibody / immunosensor / Langmuir Blodgett technique / atomic force microscopy / surface plasmon resonance
Paper # CPM96-34,OME96-21
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Committee CPM
Conference Date 1996/6/21(1days)
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Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Fabrication of Optical Biosensor Based on LB Films of Biosynthetically Lipid-Tagged Antibody
Sub Title (in English)
Keyword(1) antibody
Keyword(2) immunosensor
Keyword(3) Langmuir Blodgett technique
Keyword(4) atomic force microscopy
Keyword(5) surface plasmon resonance
1st Author's Name Yoshiki Hirata
1st Author's Affiliation AIST, National Institute of Bioscience and Human-Technology()
2nd Author's Name Marja-Leena Laukkanen
2nd Author's Affiliation VTT Biotechnology and Food Research
3rd Author's Name Kari Keinanen
3rd Author's Affiliation VTT Biotechnology and Food Research
4th Author's Name Etsuo Shinohara
4th Author's Affiliation Olympus Optics Co. Ltd.
5th Author's Name Fumio Mizutani
5th Author's Affiliation AIST, National Institute of Bioscience and Human-Technology
6th Author's Name Tasuo Katsura
6th Author's Affiliation AIST, National Institute of Bioscience and Human-Technology
7th Author's Name Masuo Aizawa
7th Author's Affiliation Tokyo Institute of Technology, Biomolecular Engineering
Date 1996/6/21
Paper # CPM96-34,OME96-21
Volume (vol) vol.96
Number (no) 121
Page pp.pp.-
#Pages 6
Date of Issue