| Presentation | 2000/11/23 Gene extraction of Escherichia coli O157 by the metal coating hollow fiber membrane Sadayuki FUNAKI, Makoto EBIKAWA, Koichi KIMURA, Kazuyuki KIMURA, Kenji MISAWA, Junji ARISAWA, Osamu IGARASHI, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have reported that the gene was effectively extracted by the metal coating hollow fiber membrane. In this report, we showed the new method for the gene extraction using the electrophoresis. Escherichia coli O157 was trapped on the metal coating hollow fiber membrance. After the cell membrane of E.coli was lysed chemically, the E.coli O157 gene was extracted by electrophoresis. As result, the vero toxin 1-gene of E.coli O157 was detected from stool samples contained 4×10^5 of E.coli O157. Therefore, It is possible to apply this gene extraction system to the examination in the early stage of the E.coli O157 gene. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Metal coating hollow fiber membrane / Gene extraction / E.coliO157 |
| Paper # | MBE2000-88 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2000/11/23(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Gene extraction of Escherichia coli O157 by the metal coating hollow fiber membrane |
| Sub Title (in English) | |
| Keyword(1) | Metal coating hollow fiber membrane |
| Keyword(2) | Gene extraction |
| Keyword(3) | E.coliO157 |
| 1st Author's Name | Sadayuki FUNAKI |
| 1st Author's Affiliation | Hokkaido Institute of Technology() |
| 2nd Author's Name | Makoto EBIKAWA |
| 2nd Author's Affiliation | Hokkaido Institute of Technology |
| 3rd Author's Name | Koichi KIMURA |
| 3rd Author's Affiliation | Hokkaido Institute of Technology |
| 4th Author's Name | Kazuyuki KIMURA |
| 4th Author's Affiliation | Hokkaido Institute of Technology |
| 5th Author's Name | Kenji MISAWA |
| 5th Author's Affiliation | Hokkaido Institute of Technology |
| 6th Author's Name | Junji ARISAWA |
| 6th Author's Affiliation | Hokkaido Institute of Technology |
| 7th Author's Name | Osamu IGARASHI |
| 7th Author's Affiliation | NIX, Inc |
| Date | 2000/11/23 |
| Paper # | MBE2000-88 |
| Volume (vol) | vol.100 |
| Number (no) | 478 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |