| Presentation | 2000/10/6 Extraction of the viral gene by electrophoresis by using the metal coating hollow fiber membrane Toshitsugu SUGAWARA, Makoto EBIKAWA, Koichi KIMURA, Kazuyuki KIMURA, Kenji MISAWA, Junji ARISAWA, Osamu IGARASHI, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | A new method of the gene extraction which was important for the gene diagnosis was proposed in this paper. Herpes simplex virus was concentrated in the metal coating hollow fiber membrane which was coated with metal, and then the extraction of viral gene by electrophoresis was attempted after the viral envelope and capsid were lysed chemically. As a result, the target gene of the virus was detected from 5×10^4 TCID_<100>/ml of the viral suspension. The time which is necessary for this gene extraction was less than 10 minutes. This extraction system has a possibility that it is automated because no other apparatus are needed than the power supply and the pump. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Metal coating hollow fiber membrane / Gene extraction / Electrophoresis |
| Paper # | MBE2000-76 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 2000/10/6(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Extraction of the viral gene by electrophoresis by using the metal coating hollow fiber membrane |
| Sub Title (in English) | |
| Keyword(1) | Metal coating hollow fiber membrane |
| Keyword(2) | Gene extraction |
| Keyword(3) | Electrophoresis |
| 1st Author's Name | Toshitsugu SUGAWARA |
| 1st Author's Affiliation | Hokkaido Institute of Technology() |
| 2nd Author's Name | Makoto EBIKAWA |
| 2nd Author's Affiliation | Hokkaido Institute of Technology |
| 3rd Author's Name | Koichi KIMURA |
| 3rd Author's Affiliation | Hokkaido Institute of Technology |
| 4th Author's Name | Kazuyuki KIMURA |
| 4th Author's Affiliation | Hokkaido Institute of Technology |
| 5th Author's Name | Kenji MISAWA |
| 5th Author's Affiliation | Hokkaido Institute of Technology |
| 6th Author's Name | Junji ARISAWA |
| 6th Author's Affiliation | Hokkaido Institute of Technology |
| 7th Author's Name | Osamu IGARASHI |
| 7th Author's Affiliation | NIX, Inc. |
| Date | 2000/10/6 |
| Paper # | MBE2000-76 |
| Volume (vol) | vol.100 |
| Number (no) | 350 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |