| Presentation | 1999/7/15 Spatial comparison of micromyocardial metabolic activity and microvascular blood flow distribution by NADH fluorescent imaging and molecular flow tracer imaging method Tatsuya Kajita, Yasuo Ogasawara, Takeshi Matsumoto, Hiroyuki Tachibana, Hiroshi Nakamoto, Seiichi Mochizuki, Fumiyuki Shigetou, Fumihiko Kajiya, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Our aims were to visualize myocardial NADH fluorescences image reflecting the mitochondrial metabolism and to compare the obtained images with the corresponding myocardial blood flow distribution in isolated, perfused rat hearts (Langendorff perfusion mode). We developed a fluorescence CCD system, consisting of an optical lens system with a barrier filter for NADH fluorescence, an ultraviolet light (370 nm) source, and a CCD video camera. Microvascular blood flow distribution was imaged by quantitative autoradiography combined with ^3H-labeled desmethylimipramine (^3H-DMI) deposition technique. Myocardial metabolic activity showed spatial heterogeneity, the size of which is nearly identical to the minimum circulatory units (300-500μm) and is closely related with blood flow distribution in the myocardial microcirculation during hypoxic state. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | isolated rat hearts / CCD / mitochondrial NADH fluorescence / molecular flow tracer / hypoxia |
| Paper # | MBE99-52 |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 1999/7/15(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
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| Paper Information | |
| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Spatial comparison of micromyocardial metabolic activity and microvascular blood flow distribution by NADH fluorescent imaging and molecular flow tracer imaging method |
| Sub Title (in English) | |
| Keyword(1) | isolated rat hearts |
| Keyword(2) | CCD |
| Keyword(3) | mitochondrial NADH fluorescence |
| Keyword(4) | molecular flow tracer |
| Keyword(5) | hypoxia |
| 1st Author's Name | Tatsuya Kajita |
| 1st Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School() |
| 2nd Author's Name | Yasuo Ogasawara |
| 2nd Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 3rd Author's Name | Takeshi Matsumoto |
| 3rd Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 4th Author's Name | Hiroyuki Tachibana |
| 4th Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 5th Author's Name | Hiroshi Nakamoto |
| 5th Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 6th Author's Name | Seiichi Mochizuki |
| 6th Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 7th Author's Name | Fumiyuki Shigetou |
| 7th Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| 8th Author's Name | Fumihiko Kajiya |
| 8th Author's Affiliation | Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School |
| Date | 1999/7/15 |
| Paper # | MBE99-52 |
| Volume (vol) | vol.99 |
| Number (no) | 177 |
| Page | pp.pp.- |
| #Pages | 8 |
| Date of Issue | |