| Presentation | 1998/5/29 High efficiency transformation of fission yeast treated with thiol compounds by electroporation Minoru Suga, Koutarou Aoyam, Masaharu Isobe, Toyomasa Hatakeyama, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | a high efficiency method of Schizosaccharomyces pombe transformation by electroporation has been developed. In pretreatment with thiol compounds before the pulse application, the cells grown on a synthetic medium (SD) instead of a natural medium (YPD) drastically improved a transformation efficiency. The transformation efficiency of this protocol was approximately 10-fold higher than that of conventional treatment without Dithiothreitol. The best efficiency, approximately 1.2×10^7 transformants per μg of DNA, was achieved in the case of electric field 11kV/cm and its time constant 5 msec. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Electroporation / Transformation / Thiol compounds / Synthetic medium / Fission yeast / Electric field |
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| Conference Information | |
| Committee | MBE |
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| Conference Date | 1998/5/29(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Registration To | ME and Bio Cybernetics (MBE) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | High efficiency transformation of fission yeast treated with thiol compounds by electroporation |
| Sub Title (in English) | |
| Keyword(1) | Electroporation |
| Keyword(2) | Transformation |
| Keyword(3) | Thiol compounds |
| Keyword(4) | Synthetic medium |
| Keyword(5) | Fission yeast |
| Keyword(6) | Electric field |
| 1st Author's Name | Minoru Suga |
| 1st Author's Affiliation | Faculty of Engineering, Toyama University() |
| 2nd Author's Name | Koutarou Aoyam |
| 2nd Author's Affiliation | Faculty of Engineering, Toyama University |
| 3rd Author's Name | Masaharu Isobe |
| 3rd Author's Affiliation | Faculty of Engineering, Toyama University |
| 4th Author's Name | Toyomasa Hatakeyama |
| 4th Author's Affiliation | Faculty of Engineering, Toyama University |
| Date | 1998/5/29 |
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| Volume (vol) | vol.98 |
| Number (no) | 94 |
| Page | pp.pp.- |
| #Pages | 5 |
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