Presentation 1994/5/28
Electrofusion of yeast cells and electric effect on their culture
Minoru Suga, Toyomasa Hatakeyama,
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Abstract(in Japanese) (See Japanese page)
Abstract(in English) In order to continue electrofusion of yeast cells and their culture,we used solution with 10% glucose in both the cases.The yeast protoplasts could be formed the pearl chains,when AC electric field of 1MHz with 10kV, m was applied.The most high ratio of the fused cells(about 10%)could be gotten by use of two DC pulses with peak strength of 700kV/m and time constant of 40μ s ec at interval of about 1sec.When DC field with 100V/m was applied yeasts in culture,they were not formed near both the eletrodes, because of pH change.
Keyword(in Japanese) (See Japanese page)
Keyword(in English) Electrofusion / Yeast / Electric Field / Cell Culture
Paper # MBE94-23
Date of Issue

Conference Information
Committee MBE
Conference Date 1994/5/28(1days)
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Paper Information
Registration To ME and Bio Cybernetics (MBE)
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Electrofusion of yeast cells and electric effect on their culture
Sub Title (in English)
Keyword(1) Electrofusion
Keyword(2) Yeast
Keyword(3) Electric Field
Keyword(4) Cell Culture
1st Author's Name Minoru Suga
1st Author's Affiliation Faculty of Engineering,Toyama University()
2nd Author's Name Toyomasa Hatakeyama
2nd Author's Affiliation Faculty of Engineering,Toyama University
Date 1994/5/28
Paper # MBE94-23
Volume (vol) vol.94
Number (no) 81
Page pp.pp.-
#Pages 6
Date of Issue