Presentation 1997/7/24
Optical imaging of spatiotemporal neural signal propagation in cultured neuronal networks
Hideo Kawaguchi, Kohyu Fukunishi,
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Abstract(in English) Two-dimensional neuronal networks were formed using a dissociated culture of rat hippocampal neurons on glass plates. Neural activity in response to pulse stimuli applied to the neurons by whole-cell clamp electrodes was observed with a 128-channel optical recording apparatus using a voltage-sensitive dye, RH482. Dendrites emerging from the somata of single neurons were classified according to two signal-transmission properties, those with action potentials and those with electrotonic potentials, by evaluating the conduction velocities of pulse responses. The distinction between these two properties seemed to be related to the morphological differences in input connectivity with the axons of neighboring neurons. Recursive neural responses were also observed in neuronal circuits involved in two-neuron systems.
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Keyword(in English) Cultured neuronal network / Optical imaging / Conduction velocity / Dendrite / Rat hippocampus
Paper # NC97-30
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Committee NC
Conference Date 1997/7/24(1days)
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Registration To Neurocomputing (NC)
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Optical imaging of spatiotemporal neural signal propagation in cultured neuronal networks
Sub Title (in English)
Keyword(1) Cultured neuronal network
Keyword(2) Optical imaging
Keyword(3) Conduction velocity
Keyword(4) Dendrite
Keyword(5) Rat hippocampus
1st Author's Name Hideo Kawaguchi
1st Author's Affiliation Advanced Research Laboratory, Hitachi, Ltd.()
2nd Author's Name Kohyu Fukunishi
2nd Author's Affiliation Advanced Research Laboratory, Hitachi, Ltd.
Date 1997/7/24
Paper # NC97-30
Volume (vol) vol.97
Number (no) 201
Page pp.pp.-
#Pages 6
Date of Issue