Presentation 2000/5/4
Analysis of DNA Microarray Images
Naoya Ohta, Youhei Hachisu, Tatsuya Akutsu, Asao Fujiyama,
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Abstract(in English) DNA micorarray is known as a method to obtain information of many different genes by one experiment. In order to effectively use this method, however. we need to automate the image processing part of the method. We describe an image processing algorithm for this purpose. A DNA micorarray image consists of a lot of spots regularly placed in a grid shape. The intensity of each spot corresponds to activeness of a specific gene. Therefore, the function of the image processing algorithm is to identify each spot and to measure its intensity. The algorithm first detects candidates of the spots and determines the angle and position of the grid formed by the spots. Then, it selects correct spots from the candidates using the information of the grid position. Next, the algorithm estimates the background levels of the image by intensity histograms and measure the intensity of each spot.
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Keyword(in English) DNA microarray / gene network / spot detection / line detection / background level estimation
Paper # PRMU2000-9, MI2000-9
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Committee MI
Conference Date 2000/5/4(1days)
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Registration To Medical Imaging (MI)
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) Analysis of DNA Microarray Images
Sub Title (in English)
Keyword(1) DNA microarray
Keyword(2) gene network
Keyword(3) spot detection
Keyword(4) line detection
Keyword(5) background level estimation
1st Author's Name Naoya Ohta
1st Author's Affiliation Gunma University()
2nd Author's Name Youhei Hachisu
2nd Author's Affiliation Gunma University
3rd Author's Name Tatsuya Akutsu
3rd Author's Affiliation University of Tokyo
4th Author's Name Asao Fujiyama
4th Author's Affiliation National Institute of Genetics
Date 2000/5/4
Paper # PRMU2000-9, MI2000-9
Volume (vol) vol.100
Number (no) 45
Page pp.pp.-
#Pages 8
Date of Issue