| Presentation | 2003/6/19 Synthesis and analysis of a master pattern of RLGS profiles of genomic DNA Masayuki NAKAZAWA, Masao KIDANI, Yasuo WATANABE, Katsutoshi TAKAHASHI, |
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| PDF Download Page | PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | A pattern analysis system of two-dimensional gel electrophoresis images based on RLGS (Restriction Landmark Genomic Scanning) method has been developed. It employs direct end-labeling of the genomic DNA fragments cut by two or three kinds of restriction enzyme and, separates them by means of 2-D gel electrophoresis. For the purpose of gene diagnosis in the next stage, we propose an automated synthesis method of the common pattern of multiple RLGS profiles for two sets; one comes from cancer cells, the other from normal, these are already classified by a clinician. The common pattern, which we call it 'master pattern', is generated by using the coordinates and density of all spots. As the master pattern is characteristic to a variety of diseases or depends on some gene expression, we can apply it to a powerful tools for gene diagnosis. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | RLGS(Restriction Landmark Genomic Scanning) method / Image analysis / Gene diagnosis / Master pattern Delauney net |
| Paper # | PRMU2003-28 |
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| Conference Information | |
| Committee | PRMU |
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| Conference Date | 2003/6/19(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
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| Paper Information | |
| Registration To | Pattern Recognition and Media Understanding (PRMU) |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Synthesis and analysis of a master pattern of RLGS profiles of genomic DNA |
| Sub Title (in English) | |
| Keyword(1) | RLGS(Restriction Landmark Genomic Scanning) method |
| Keyword(2) | Image analysis |
| Keyword(3) | Gene diagnosis |
| Keyword(4) | Master pattern Delauney net |
| 1st Author's Name | Masayuki NAKAZAWA |
| 1st Author's Affiliation | Kanazawa Technical College() |
| 2nd Author's Name | Masao KIDANI |
| 2nd Author's Affiliation | Kanazawa Institute of Technology |
| 3rd Author's Name | Yasuo WATANABE |
| 3rd Author's Affiliation | Kanazawa Institute of Technology |
| 4th Author's Name | Katsutoshi TAKAHASHI |
| 4th Author's Affiliation | National Institute of Advanced Industrial Science and Technology |
| Date | 2003/6/19 |
| Paper # | PRMU2003-28 |
| Volume (vol) | vol.103 |
| Number (no) | 150 |
| Page | pp.pp.- |
| #Pages | 6 |
| Date of Issue | |