| Presentation | 2018-01-27 Ru錯体による培養細胞組織中の酸素濃度勾配イメージング法の検討 Kaima Okubo, Yusuke Tsuruno, Takahiro Fujiwara, Umihiro Inoue, Yoshihisa Yamaoka, Eiji Takahashi, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | To investigate the possibility that tumor cells migrate toward intratumor blood vessels according to the gradient of oxygen concentration in tissue, we devised the gap cover glass (GCG) that allows to produce gradients of oxygen in the monolayer cultured cells. To verify the oxygen gradients, we conducted oxygen imaging in the extracellular medium in cultured MDA-MB-231 and COS-7 cells using a ruthenium complex under conditions of different cell density, cell species, or cellular respiration, all of which significantly alter the oxygen consumption rate per unit volume of the cell. The measured oxygen gradients were consistent with the changes in the oxygen consumption rate. Thus, we conclude that the GCG produces gradients of oxygen in monolayer cultured cells. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | tumor cells / oxygen gradients / migration / oxygen imaging |
| Paper # | MBE2017-75 |
| Date of Issue | 2018-01-19 (MBE) |
| Conference Information | |
| Committee | MBE / NC / NLP |
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| Conference Date | 2018/1/26(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kyushu Institute of Technology |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | ME, generalImplementation of Neuro Computing,Analysis and Modeling of Human Science, |
| Chair | Kazuki Nakajima(Univ. of Toyama) / Masafumi Hagiwara(Keio Univ.) / Masaharu Adachi(Tokyo Denki Univ.) |
| Vice Chair | Masaki Kyoso(TCU) / Yutaka Hirata(Chubu Univ.) / Norikazu Takahashi(Okayama Univ.) |
| Secretary | Masaki Kyoso(Toyama Pref. Univ.) / Yutaka Hirata(Kindai Univ.) / Norikazu Takahashi(Tokyo Inst. of Tech.) |
| Assistant | Kim Juhyon(Univ. of Toyama) / Takumi Kobayashi(YNU) / Yoshihisa Shinozawa(Keio Univ.) / Keiichiro Inagaki(Chubu Univ.) / Toshihiro Tachibana(Shonan Inst. of Tech.) / Masayuki Kimura(Kyoto Univ.) |
| Paper Information | |
| Registration To | Technical Committee on ME and Bio Cybernetics / Technical Committee on Neurocomputing / Technical Committee on Nonlinear Problems |
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| Language | JPN-ONLY |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | |
| Sub Title (in English) | |
| Keyword(1) | tumor cells |
| Keyword(2) | oxygen gradients |
| Keyword(3) | migration |
| Keyword(4) | oxygen imaging |
| 1st Author's Name | Kaima Okubo |
| 1st Author's Affiliation | Saga University(Saga Univ.) |
| 2nd Author's Name | Yusuke Tsuruno |
| 2nd Author's Affiliation | Saga University(Saga Univ.) |
| 3rd Author's Name | Takahiro Fujiwara |
| 3rd Author's Affiliation | Saga University(Saga Univ.) |
| 4th Author's Name | Umihiro Inoue |
| 4th Author's Affiliation | Saga University(Saga Univ.) |
| 5th Author's Name | Yoshihisa Yamaoka |
| 5th Author's Affiliation | Saga University(Saga Univ.) |
| 6th Author's Name | Eiji Takahashi |
| 6th Author's Affiliation | Saga University(Saga Univ.) |
| Date | 2018-01-27 |
| Paper # | MBE2017-75 |
| Volume (vol) | vol.117 |
| Number (no) | MBE-416 |
| Page | pp.pp.47-52(MBE), |
| #Pages | 6 |
| Date of Issue | 2018-01-19 (MBE) |