| Presentation | 2018-01-27 Study on purity required for preparation process of photoswitch molecule (BENAQ) by cytotoxicity test of Nitroazobenzene S1 Yurina Yoshimura, Haruna Doi, Yasuo Terasawa, Hiroyuki Tashiro, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Recently, optical nerve stimulation is intensively developed and it is expected to realize high-density nerve stimulation which is difficult by electrical stimulation. Photoswitch with photoactive molecule called BENAQ or DENAQ provides photoresponsiveness to nerve cells. In this study, we performed cytotoxicity test of Nitroazobenzene S1 as a starting substance used for synthesis of BENAQ, and then investigate purity required for the BENAQ synthesis process. In the culture with 10 μmol/L-Nitroazobenzene S1 in the medium, the number of colony formation significantly decreased compared with it cultured only with medium, at significance level 5%. Therefore, it is necessary that the residual concentration of Nitroazobenzene S1 remaining in 100 μmol/L-BENAQ as maximum effect for the retina of rd1 mouse retina is less than 10 μmol/L. For BENAQ synthesis, purity of 90% or more is required for purification of Nitroazobenzene S1. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | V79 Cell / Cytotoxicity Test / Preparative Chromatography / Optical Nerve Stimulation / BENAQ |
| Paper # | MBE2017-77 |
| Date of Issue | 2018-01-19 (MBE) |
| Conference Information | |
| Committee | MBE / NC / NLP |
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| Conference Date | 2018/1/26(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kyushu Institute of Technology |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | ME, generalImplementation of Neuro Computing,Analysis and Modeling of Human Science, |
| Chair | Kazuki Nakajima(Univ. of Toyama) / Masafumi Hagiwara(Keio Univ.) / Masaharu Adachi(Tokyo Denki Univ.) |
| Vice Chair | Masaki Kyoso(TCU) / Yutaka Hirata(Chubu Univ.) / Norikazu Takahashi(Okayama Univ.) |
| Secretary | Masaki Kyoso(Toyama Pref. Univ.) / Yutaka Hirata(Kindai Univ.) / Norikazu Takahashi(Tokyo Inst. of Tech.) |
| Assistant | Kim Juhyon(Univ. of Toyama) / Takumi Kobayashi(YNU) / Yoshihisa Shinozawa(Keio Univ.) / Keiichiro Inagaki(Chubu Univ.) / Toshihiro Tachibana(Shonan Inst. of Tech.) / Masayuki Kimura(Kyoto Univ.) |
| Paper Information | |
| Registration To | Technical Committee on ME and Bio Cybernetics / Technical Committee on Neurocomputing / Technical Committee on Nonlinear Problems |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Study on purity required for preparation process of photoswitch molecule (BENAQ) by cytotoxicity test of Nitroazobenzene S1 |
| Sub Title (in English) | |
| Keyword(1) | V79 Cell |
| Keyword(2) | Cytotoxicity Test |
| Keyword(3) | Preparative Chromatography |
| Keyword(4) | Optical Nerve Stimulation |
| Keyword(5) | BENAQ |
| 1st Author's Name | Yurina Yoshimura |
| 1st Author's Affiliation | Kyushu University(kyushu Univ.) |
| 2nd Author's Name | Haruna Doi |
| 2nd Author's Affiliation | Kyushu University(kyushu Univ.) |
| 3rd Author's Name | Yasuo Terasawa |
| 3rd Author's Affiliation | NIDEK CO., LTD.(NIDEK) |
| 4th Author's Name | Hiroyuki Tashiro |
| 4th Author's Affiliation | Kyushu University(kyushu Univ.) |
| Date | 2018-01-27 |
| Paper # | MBE2017-77 |
| Volume (vol) | vol.117 |
| Number (no) | MBE-416 |
| Page | pp.pp.59-64(MBE), |
| #Pages | 6 |
| Date of Issue | 2018-01-19 (MBE) |