| Presentation | 2017-06-27 Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design Tomoo Sato, Hiromasa Yamashita, Yoshitaka Miyamoto, Kazuo Kurokawa, Toshio Chiba, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | In order to investigate the gene incorporation into cell by sonoporation using the optical microscope, the irradiation conditions of ultrsound were considered. The cultured cells (HepG2) with plasmid DNA (pEGFP-N3) and microbubbles (sonazoid), were exposure to the ultrasound. And the fluorescence intensity from Green Fluorescent Protein which was developed was measured. We assigned the following four factors to L9-orthogonal table of an experimental design and analyzed the factors which induce large the changes of the fluorescence intensity. [ (1) distance between ultrasound transmitter and bottom of microplate where the cells are cultured (2) intensity of exposing ultrasound, (3) exposing period, (4) duty ratio of exposing ultrasound ] The intensity of fluorescence of the GFP was shown to be influenced by the distance and the intensity of ultrasound. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Fetal treatment, / Transgenesis / Sonoporation / Ultrasound / Microbubble / Experimental design |
| Paper # | US2017-24 |
| Date of Issue | 2017-06-20 (US) |
| Conference Information | |
| Committee | US |
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| Conference Date | 2017/6/27(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | Minoru Kurosawa(Tokyo Inst. of Tech.) |
| Vice Chair | Nobuyuki Endoh(KU) / Shinichi Takeuchi(Toin Univ. of Yokohama) |
| Secretary | Nobuyuki Endoh(Univ. of Toyama) / Shinichi Takeuchi(Univ. of Tokyo) |
| Assistant | Takenobu Tsuchiya(KU) |
| Paper Information | |
| Registration To | Technical Committee on Ultrasonics |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design |
| Sub Title (in English) | |
| Keyword(1) | Fetal treatment, |
| Keyword(2) | Transgenesis |
| Keyword(3) | Sonoporation |
| Keyword(4) | Ultrasound |
| Keyword(5) | Microbubble |
| Keyword(6) | Experimental design |
| 1st Author's Name | Tomoo Sato |
| 1st Author's Affiliation | Port and Airport Research Institute(PARI) |
| 2nd Author's Name | Hiromasa Yamashita |
| 2nd Author's Affiliation | Nihon University(Nihon Univ.) |
| 3rd Author's Name | Yoshitaka Miyamoto |
| 3rd Author's Affiliation | Nagoya University(Nagoya Univ.) |
| 4th Author's Name | Kazuo Kurokawa |
| 4th Author's Affiliation | Institute of Physical and Chemical Research(RIKEN) |
| 5th Author's Name | Toshio Chiba |
| 5th Author's Affiliation | Nihon University(Nihon Univ.) |
| Date | 2017-06-27 |
| Paper # | US2017-24 |
| Volume (vol) | vol.117 |
| Number (no) | US-111 |
| Page | pp.pp.19-23(US), |
| #Pages | 5 |
| Date of Issue | 2017-06-20 (US) |