| Presentation | 2017-04-21 Effect of Protein Adsorption Layers on Preparation of Hydroxyapatite-Coated Polystyrene Substrates Based on Biomimetic Process Kazutoshi Iijima, Ayako Iizuka, Ryo Suzuki, Hitomi Ueno-Yokohata, Nobutaka Kiyokawa, Mineo Hashizume, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have developed a method to functionalize polystyrene (PS) substrate surfaces with hydroxyapatite (HAp) via protein adsorption layers such as human serum albumin (HSA) in simulated body fluid (SBF). In this study, the effect of protein adsorption layers on HAp deposition on PS substrate surfaces in SBF was evaluated using HSA, type I collagen, hen egg white lysozyme, and poly l-glutamic acid, an acidic protein analogue. Pretreatments with alternate soaking process (ASP) using solutions containing calcium ions and phosphate ions followed by incubation with SBF for 24 h resulted in HAp deposition on PS plates with protein adsorption layers, and the deposition behaviors were correlated with adsorption ability and charge state of proteins. Adhesion, proliferation, and differentiation of mesenchymal stem cells on HAp-coated PS plates were also investigated. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Hydroxyapatite / Simulated Body Fluid / Polystyrene / Protein / Mesenchymal Stem Cell |
| Paper # | SDM2017-7,OME2017-7 |
| Date of Issue | 2017-04-13 (SDM, OME) |
| Conference Information | |
| Committee | OME / SDM |
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| Conference Date | 2017/4/20(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tatsugochou Shougaigakushuu Center |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | Organic molecular devices, silicon device, biotechnology, thin film device, novel material, evaluation method, etc |
| Chair | Naoki Matsuda(AIST) / Tatsuya Kunikiyo(Renesas) |
| Vice Chair | Tatsuo Mori(Aichi Inst. of Tech.) / Takahiro Shinada(Tohoku Univ.) |
| Secretary | Tatsuo Mori(NTT) / Takahiro Shinada(Univ. of Tokyo) |
| Assistant | Hirotake Kajii(Osaka Univ.) / Dai Taguchi(Tokyo Inst. of Tech.) / Hiroya Ikeda(Shizuoka Univ.) |
| Paper Information | |
| Registration To | Technical Committee on Organic Molecular Electronics / Technical Committee on Silicon Device and Materials |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Effect of Protein Adsorption Layers on Preparation of Hydroxyapatite-Coated Polystyrene Substrates Based on Biomimetic Process |
| Sub Title (in English) | |
| Keyword(1) | Hydroxyapatite |
| Keyword(2) | Simulated Body Fluid |
| Keyword(3) | Polystyrene |
| Keyword(4) | Protein |
| Keyword(5) | Mesenchymal Stem Cell |
| 1st Author's Name | Kazutoshi Iijima |
| 1st Author's Affiliation | Tokyo University of Science(Tokyo Univ. of Sci.) |
| 2nd Author's Name | Ayako Iizuka |
| 2nd Author's Affiliation | Tokyo University of Science(Tokyo Univ. of Sci.) |
| 3rd Author's Name | Ryo Suzuki |
| 3rd Author's Affiliation | Tokyo University of Science(Tokyo Univ. of Sci.) |
| 4th Author's Name | Hitomi Ueno-Yokohata |
| 4th Author's Affiliation | National Research Institute for Child Health and Development(NCCHD) |
| 5th Author's Name | Nobutaka Kiyokawa |
| 5th Author's Affiliation | National Research Institute for Child Health and Development(NCCHD) |
| 6th Author's Name | Mineo Hashizume |
| 6th Author's Affiliation | Tokyo University of Science(Tokyo Univ. of Sci.) |
| Date | 2017-04-21 |
| Paper # | SDM2017-7,OME2017-7 |
| Volume (vol) | vol.117 |
| Number (no) | SDM-7,OME-8 |
| Page | pp.pp.31-34(SDM), pp.31-34(OME), |
| #Pages | 4 |
| Date of Issue | 2017-04-13 (SDM, OME) |