| Presentation | 2017-03-14 Estimation of refractive index in the living brain by optimizing spherical aberration. Hiromu Monai, Yoshihiro Ue, Kaori Higuchi, Daisuke Nishiwaki, Tetsuya Tajima, Kenya Okazaki, Hiroshi Hama, Hirase Hajime, Atsushi Miyawaki, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | The high-resolution imaging of fine structures such as neurites requires objectives with high numerical apertures (NAs). However, spherical aberration (SA) due to the mismatch of refractive indices between an immersion medium and samples, often distorts acquired images. Whereas a correction collar attached to high NAs objectives has been designed to compensate SA, its manual adjustment and acquisition of the best image of a given samples is the most difficult mission. To solve the problem, we have developed SA compensation system, by motorizing collection collar and formulating an algorithm which calculates the optimum collection collar position based on contrasts of images. This system allows quantitative analysis of fine structures of cerebral cortex in vivo. Furthermore, as this system enables us to estimate the refractive index of a given samples on the basis of the compensation degree, we found the cortical structure could be optically heterogeneous along the z-axis in the living brain. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | correction collar / refractive index (RI) / in vivo imaging / two-photon microscopy |
| Paper # | NC2016-93 |
| Date of Issue | 2017-03-06 (NC) |
| Conference Information | |
| Committee | MBE / NC |
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| Conference Date | 2017/3/13(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | Yutaka Fukuoka(Kogakuin Univ.) / Shigeo Sato(Tohoku Univ.) |
| Vice Chair | Kazuki Nakajima(Univ. of Toyama) / Masafumi Hagiwara(Keio Univ.) |
| Secretary | Kazuki Nakajima(Kogakuin Univ.) / Masafumi Hagiwara(Toyama Pref. Univ.) |
| Assistant | Ryota Horie(Shibaura Inst. of Tech.) / Kim Juhyon(Univ. of Toyama) / Hisanao Akima(Tohoku Univ.) / Yoshihisa Shinozawa(Keio Univ.) |
| Paper Information | |
| Registration To | Technical Committee on ME and Bio Cybernetics / Technical Committee on Neurocomputing |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Estimation of refractive index in the living brain by optimizing spherical aberration. |
| Sub Title (in English) | |
| Keyword(1) | correction collar |
| Keyword(2) | refractive index (RI) |
| Keyword(3) | in vivo imaging |
| Keyword(4) | two-photon microscopy |
| 1st Author's Name | Hiromu Monai |
| 1st Author's Affiliation | RIKEN(RIKEN) |
| 2nd Author's Name | Yoshihiro Ue |
| 2nd Author's Affiliation | .RIKEN/OLYMPUS Corp(RIKEN/OLYMPUS) |
| 3rd Author's Name | Kaori Higuchi |
| 3rd Author's Affiliation | .RIKEN/OLYMPUS Corp(RIKEN/OLYMPUS) |
| 4th Author's Name | Daisuke Nishiwaki |
| 4th Author's Affiliation | .RIKEN/OLYMPUS Corp(RIKEN/OLYMPUS) |
| 5th Author's Name | Tetsuya Tajima |
| 5th Author's Affiliation | .RIKEN/OLYMPUS Corp(RIKEN/OLYMPUS) |
| 6th Author's Name | Kenya Okazaki |
| 6th Author's Affiliation | .RIKEN/OLYMPUS Corp(RIKEN/OLYMPUS) |
| 7th Author's Name | Hiroshi Hama |
| 7th Author's Affiliation | RIKEN(RIKEN) |
| 8th Author's Name | Hirase Hajime |
| 8th Author's Affiliation | RIKEN(RIKEN) |
| 9th Author's Name | Atsushi Miyawaki |
| 9th Author's Affiliation | RIKEN(RIKEN) |
| Date | 2017-03-14 |
| Paper # | NC2016-93 |
| Volume (vol) | vol.116 |
| Number (no) | NC-521 |
| Page | pp.pp.171-176(NC), |
| #Pages | 6 |
| Date of Issue | 2017-03-06 (NC) |