| Presentation | 2017-01-19 Effect of reaction conditions on magnetic signal from magnetic markers in liquid-phase immunoassays Kota Nakamura, Yoshitsugu Takafuji, Kota Irie, Teruyoshi Sasayama, Takashi Yoshida, Keiji Enpuku, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have been developing a highly sensitive liquid-phase detection of biological targets using magnetic markers and HTS SQUID. In order to realize high sensitivity of this technique, it is necessary to decrease signal from free markers, which we call blank signal, as well as to increase signal from bound markers. Previously, we showed that dispersion process after magnetization is effective to decrease blank signal. In this study, we optimized reaction conditions when the dispersion process was introduced, i.e., density of magnetic markers and time used for reaction. We also measured diameter of magnetic markers before and after the magnetization so as to study the aggregation caused by the magnetic field. It was found that magnetic field accelerated aggregation of magnetic markers. Then, we calculated blank signal from the agglomerate of free markers by taking into account their Brownian relaxation. The calculated blank signal reasonably agreed with the experimental result. Finally, we measured the Brownian relaxation time of the bound markers, and found that the measured time was shorter than the theoretical expectation. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Biosensor / Brownian Relaxation / Immunoassay / Magnetic Marker / HTS SQUID |
| Paper # | SCE2016-46 |
| Date of Issue | 2017-01-12 (SCE) |
| Conference Information | |
| Committee | SCE |
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| Conference Date | 2017/1/19(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | Nobuyuki Yoshikawa(Yokohama National Univ.) |
| Vice Chair | |
| Secretary | (National Defense Academy) |
| Assistant | Hiroyuki Akaike(Nagoya Univ.) / Yuki Yamanashi(Yokohama National Univ.) |
| Paper Information | |
| Registration To | Technical Committee on Superconductive Electronics |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Effect of reaction conditions on magnetic signal from magnetic markers in liquid-phase immunoassays |
| Sub Title (in English) | |
| Keyword(1) | Biosensor |
| Keyword(2) | Brownian Relaxation |
| Keyword(3) | Immunoassay |
| Keyword(4) | Magnetic Marker |
| Keyword(5) | HTS SQUID |
| 1st Author's Name | Kota Nakamura |
| 1st Author's Affiliation | Kyushu University(Kyushu Univ.) |
| 2nd Author's Name | Yoshitsugu Takafuji |
| 2nd Author's Affiliation | Kyushu University(Kyushu Univ.) |
| 3rd Author's Name | Kota Irie |
| 3rd Author's Affiliation | Kyushu University(Kyushu Univ.) |
| 4th Author's Name | Teruyoshi Sasayama |
| 4th Author's Affiliation | Kyushu University(Kyushu Univ.) |
| 5th Author's Name | Takashi Yoshida |
| 5th Author's Affiliation | Kyushu University(Kyushu Univ.) |
| 6th Author's Name | Keiji Enpuku |
| 6th Author's Affiliation | Kyushu University(Kyushu Univ.) |
| Date | 2017-01-19 |
| Paper # | SCE2016-46 |
| Volume (vol) | vol.116 |
| Number (no) | SCE-410 |
| Page | pp.pp.43-48(SCE), |
| #Pages | 6 |
| Date of Issue | 2017-01-12 (SCE) |