| Presentation | 2016-03-22 Basic studies on effectiveness of sonoporation for antigen transduction to dendritic cells Ryo Takada, Yusuke Oda, Ryo Suzuki, Nobuki Kudo, |
|---|---|
| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | We have been studying a sonoporation technique induced by exposure of one-shot short-pulsed ultrasound to cells with attached microbubbles (MBs). This study was carried out to show the effectiveness of the technique for cancer immune-therapy. Effectiveness of sonoporation was investigated using MBs coated by fluorescence dye placed at two typical conditions of (1) MBs attached to a cell membrane and of (2) MBs phagocytized inside a cell. In situ confocal observation showed transduction rates of 5% and 79% under each condition, respectively, suggesting the importance to optimize sonoporation conditions for cells with phagocytized MBs. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | sonoporation / dendritic cell / immune-therapy / ultrasound / bubble liposome |
| Paper # | MBE2015-109 |
| Date of Issue | 2016-03-15 (MBE) |
| Conference Information | |
| Committee | MBE / NC |
|---|---|
| Conference Date | 2016/3/22(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tamagawa University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Chair | Tetsuo Kobayashi(Kyoto Univ.) / Toshimichi Saito(Hosei Univ.) |
| Vice Chair | Yutaka Fukuoka(Kogakuin Univ.) / Shigeo Sato(Tohoku Univ.) |
| Secretary | Yutaka Fukuoka(akita noken) / Shigeo Sato(Kogakuin Univ.) |
| Assistant | Takenori Oida(Kyoto Univ.) / Ryota Horie(Shibaura Inst. of Tech.) / Hiroyuki Kanbara(Tokyo Inst. of Tech.) / Hisanao Akima(Tohoku Univ.) |
| Paper Information | |
| Registration To | Technical Committee on ME and Bio Cybernetics / Technical Committee on Neurocomputing |
|---|---|
| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Basic studies on effectiveness of sonoporation for antigen transduction to dendritic cells |
| Sub Title (in English) | |
| Keyword(1) | sonoporation |
| Keyword(2) | dendritic cell |
| Keyword(3) | immune-therapy |
| Keyword(4) | ultrasound |
| Keyword(5) | bubble liposome |
| 1st Author's Name | Ryo Takada |
| 1st Author's Affiliation | Hokkaido University(Hokkaido Univ.) |
| 2nd Author's Name | Yusuke Oda |
| 2nd Author's Affiliation | Teikyo University(Teikyo Univ.) |
| 3rd Author's Name | Ryo Suzuki |
| 3rd Author's Affiliation | Teikyo University(Teikyo Univ.) |
| 4th Author's Name | Nobuki Kudo |
| 4th Author's Affiliation | Hokkaido University(Hokkaido Univ.) |
| Date | 2016-03-22 |
| Paper # | MBE2015-109 |
| Volume (vol) | vol.115 |
| Number (no) | MBE-513 |
| Page | pp.pp.33-38(MBE), |
| #Pages | 6 |
| Date of Issue | 2016-03-15 (MBE) |