| Presentation | 2016-01-19 Magnetic Resonance Imaging using human fetuses preserved in formalin solution Mikako Kamatani, Akira Yamamoto, Reina Miyazaki, Haruyuki Makishima, Tomohisa Okada, Kaori Togashi, Shigehito Yamada, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Recently, fetal brains could have been imaged by magnetic resonance imaging (MRI) and analyzed in detail morphologically. Those images were quite fine, however, obtained by isolated brain samples, and using MRI system for experimental use therefore it is difficult to obtain enough data for fetal brain research. To facilitate the human fetal brain imaging, we tried to establish the protocol suitable for whole-body human fetuses using MRI system with 3T magnet, for clinical use. For magnetization-prepared rapid gradient-echo (MP-RAGE) sequence, no treatment were not required for fetuses in formalin solution to obtain fine images; but less signals could be obtained from the specimens in T2-weighted and diffusion-weighted imaging (DWI). To improve signals from water molecule, the fetuses were soaked in phosphate buffered saline for 3-14 days and the T2 and DWI signals were improved after the substitution. The specimens with long-term preservation in formalin solution were available for T2 and DWI as well as MP-RAGE, but tractography was not obtained from it. As next attempt, small quantity of formalin was injected into brain of new sample and the sample was soaked in PBS before imaging. As a result, clear color map and tractography was obtained by using the sample. It is required to consider method to obtain strong signal of diffusion tensor after long-time preservation. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | MRI / DTI / human fetus / congenital anomaly |
| Paper # | MI2015-100 |
| Date of Issue | 2016-01-12 (MI) |
| Conference Information | |
| Committee | MI |
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| Conference Date | 2016/1/19(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Bunka Tenbusu Kan |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | General topics in medical imaging |
| Chair | Yoshitaka Masutani(Hiroshima City Univ.) |
| Vice Chair | Yoshiki Kawata(Tokushima Univ.) / Yuichi Kimura(Kinki Univ.) |
| Secretary | Yoshiki Kawata(Aichi Inst. of Tech.) / Yuichi Kimura(Nagoya Inst. of Tech.) |
| Assistant | Ryo Haraguchi(NCVC) / Yasushi Hirano(Yamaguchi Univ.) |
| Paper Information | |
| Registration To | Technical Committee on Medical Imaging |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Magnetic Resonance Imaging using human fetuses preserved in formalin solution |
| Sub Title (in English) | |
| Keyword(1) | MRI |
| Keyword(2) | DTI |
| Keyword(3) | human fetus |
| Keyword(4) | congenital anomaly |
| 1st Author's Name | Mikako Kamatani |
| 1st Author's Affiliation | Kyoto university(Kyoto univ) |
| 2nd Author's Name | Akira Yamamoto |
| 2nd Author's Affiliation | Kyoto university(Kyoto univ) |
| 3rd Author's Name | Reina Miyazaki |
| 3rd Author's Affiliation | Kyoto university(Kyoto univ) |
| 4th Author's Name | Haruyuki Makishima |
| 4th Author's Affiliation | Kyoto university(Kyoto univ) |
| 5th Author's Name | Tomohisa Okada |
| 5th Author's Affiliation | Kyoto university(Kyoto univ) |
| 6th Author's Name | Kaori Togashi |
| 6th Author's Affiliation | Kyoto university(Kyoto univ) |
| 7th Author's Name | Shigehito Yamada |
| 7th Author's Affiliation | Kyoto university(Kyoto univ) |
| Date | 2016-01-19 |
| Paper # | MI2015-100 |
| Volume (vol) | vol.115 |
| Number (no) | MI-401 |
| Page | pp.pp.127-130(MI), |
| #Pages | 4 |
| Date of Issue | 2016-01-12 (MI) |