| Presentation | 2015-04-29 Coordination chemistry of active center of metalloenzyme studied by affinity capillary electrophoresis Nobuhiko Iki, Yosuke Sato, |
|---|---|
| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | By using affinity capillary electrophoresis, binding equilibrium of an arylsulfonamide (SA) to a series of metal-substituted carbonic anhydrase (M-CA) and kinetic stability of M-CA toward solvolysis were studied. In the binding of SA, the hydrophobic cavity of CA plays an important role to force the SA to donate the amide moiety to the metal center of CA to fulfill the tetrahedral coordination geometry. In the kinetics, the hydrophobic cavity excludes the access of EDTA to the metal center, while the tetrahedral coordination geometry supported by three histidine residues gave the Zn(II) center exceptionally high kinetic stability. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Metalloenzyme / Carbonic Anhydrase / Zinc / Affinity Capillary Electrophoresis |
| Paper # | SDM2015-3,OME2015-3 |
| Date of Issue | 2015-04-22 (SDM, OME) |
| Conference Information | |
| Committee | OME / SDM |
|---|---|
| Conference Date | 2015/4/29(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Oh-hama Nobumoto Memorial Hall |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | Thin FIlms, Functional Electronics Devices, New Functional Materials and Evaluation, BIomtechnology |
| Chair | Keizo Kato(Niigata Univ.) / Yuzou Oono(Univ. of Tsukuba) |
| Vice Chair | Naoki Matsuda(AIST) / Tatsuya Kunikiyo(Renesas) |
| Secretary | Naoki Matsuda(Canon Electronics) / Tatsuya Kunikiyo(Aichi Inst. of Tech.) |
| Assistant | Akihiro Kohno(NTT) / Shinichiro Inoue(NICT) / Tadashi Yamaguchi(Renesas) |
| Paper Information | |
| Registration To | Technical Committee on Organic Molecular Electronics / Technical Committee on Silicon Device and Materials |
|---|---|
| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Coordination chemistry of active center of metalloenzyme studied by affinity capillary electrophoresis |
| Sub Title (in English) | |
| Keyword(1) | Metalloenzyme |
| Keyword(2) | Carbonic Anhydrase |
| Keyword(3) | Zinc |
| Keyword(4) | Affinity Capillary Electrophoresis |
| 1st Author's Name | Nobuhiko Iki |
| 1st Author's Affiliation | Tohoku University(Tohoku Univ.) |
| 2nd Author's Name | Yosuke Sato |
| 2nd Author's Affiliation | Tohoku University(Tohoku Univ.) |
| Date | 2015-04-29 |
| Paper # | SDM2015-3,OME2015-3 |
| Volume (vol) | vol.115 |
| Number (no) | SDM-18,OME-19 |
| Page | pp.pp.11-14(SDM), pp.11-14(OME), |
| #Pages | 4 |
| Date of Issue | 2015-04-22 (SDM, OME) |