| Presentation | 2022-09-29 Fabrication of artificial neuronal networks for analyzing neuronal ensemble functions Hakuba Murota, Hideaki Yamamoto, Takuma Sumi, Shigeo Sato, Ayumi Hirano, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | In this study, we applied cell patterning technology using polydimethylsiloxane microfluidics to fabricate artificial neuronal networks with modular connectivity, a network topology that characterizes the mammalian cortex. Spontaneous activity of the artificial neuronal networks was recorded by fluorescence calcium imaging, and various neuronal ensemble activities were observed. Evaluation of network activity by correlation coefficients revealed that the modular patterning reduced synchrony between distant neuronal populations. Furthermore, using optogenetics technology to apply local stimulation to the artificial neuronal network, we found that evoked activity tended to be confined to the cell population at and near the point of the stimulation. These results suggest that this experimental system is useful for the functional characterization of neuronal ensembles in vitro. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Cultured Neuronal Network / Cell Patterning / Neuronal Ensembles / Optogenetics |
| Paper # | NC2022-32 |
| Date of Issue | 2022-09-22 (NC) |
| Conference Information | |
| Committee | NC / MBE |
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| Conference Date | 2022/9/29(2days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tohoku Univ. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | Brain Architecture, NC, ME |
| Chair | Hiroshi Yamakawa(Univ of Tokyo) / Junichi Hori(Niigata Univ.) |
| Vice Chair | Hirokazu Tanaka(Tokyo City Univ.) / Hisashi Yoshida(Kinki Univ.) |
| Secretary | Hirokazu Tanaka(NTT) / Hisashi Yoshida(NICT) |
| Assistant | Yoshimasa Tawatsuji(Waseda Univ.) / Tomoki Kurikawa(KMU) / Emi Yuda(Tohoku Univ) / Miki Kaneko(Osaka Univ.) |
| Paper Information | |
| Registration To | Technical Committee on Neurocomputing / Technical Committee on ME and Bio Cybernetics |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Fabrication of artificial neuronal networks for analyzing neuronal ensemble functions |
| Sub Title (in English) | |
| Keyword(1) | Cultured Neuronal Network |
| Keyword(2) | Cell Patterning |
| Keyword(3) | Neuronal Ensembles |
| Keyword(4) | Optogenetics |
| 1st Author's Name | Hakuba Murota |
| 1st Author's Affiliation | Tohoku University(Tohoku Univ.) |
| 2nd Author's Name | Hideaki Yamamoto |
| 2nd Author's Affiliation | Tohoku University(Tohoku Univ.) |
| 3rd Author's Name | Takuma Sumi |
| 3rd Author's Affiliation | Tohoku University(Tohoku Univ.) |
| 4th Author's Name | Shigeo Sato |
| 4th Author's Affiliation | Tohoku University(Tohoku Univ.) |
| 5th Author's Name | Ayumi Hirano |
| 5th Author's Affiliation | Tohoku University(Tohoku Univ.) |
| Date | 2022-09-29 |
| Paper # | NC2022-32 |
| Volume (vol) | vol.122 |
| Number (no) | NC-195 |
| Page | pp.pp.1-4(NC), |
| #Pages | 4 |
| Date of Issue | 2022-09-22 (NC) |