Presentation 2022-07-18
[Invited Talk] Evaluation of depth resolution in structured illumination microscope based on 3D-printed flexure stages
Tatsunosuke Matsui, Daigo Fujiwara,
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Abstract(in Japanese) (See Japanese page)
Abstract(in English) Recently, various types of 3D-printable optical microscopes have been reported. Many of those are open-sourced, therefore, anyone can replicate and utilize them, and moreover, anyone can contribute to the improvement of the system. Among these, the OpenFlexure Microscope families have quite unique design adopting the flexure mechanism of the 3D-printed flexible plastics, and show extremely high positioning precision combining with low-cost stepper motors. We have so far reported on the development of the structured illumination microscope (SIM) with optical sectioning capability by assembling several 3D-printed components with the OpenFlexure Delta Stage. It is theoretically known that the SIM has a depth resolution comparable with that achievable with the confocal microscopes. In our system, a depth resolution of 4.0 um has been achieved with the 0.8 NA, 60× objective lens and a grid of 50 um periodicity.
Keyword(in Japanese) (See Japanese page)
Keyword(in English) 3D print / flexure stage / structured illumination microscope / SIM / optical sectioning / depth resolution
Paper # OME2022-17
Date of Issue 2022-07-11 (OME)

Conference Information
Committee OME / IEE-DEI
Conference Date 2022/7/18(2days)
Place (in Japanese) (See Japanese page)
Place (in English) Karuizawa-machi Tourism promotion center
Topics (in Japanese) (See Japanese page)
Topics (in English) Organic film, Organic-Bio devices, etc.
Chair Toshiki Yamada(NICT) / 高橋 俊裕(電力中央研究所)
Vice Chair Eiji Itoh(Shinshu Univ.)
Secretary Eiji Itoh(Osaka Univ.) / (Tokyo Univ. of Agriculture and Tech.)
Assistant Yoshiyuki Seike(Aichi Inst. of Tech.) / Akira Baba(Niigata Univ.) / 梅本 貴弘(三菱電機) / 栗本 宗明(名古屋大学)

Paper Information
Registration To Technical Committee on Organic Molecular Electronics / Technical Meeting on Dielectrics and Electrical Insulation
Language JPN
Title (in Japanese) (See Japanese page)
Sub Title (in Japanese) (See Japanese page)
Title (in English) [Invited Talk] Evaluation of depth resolution in structured illumination microscope based on 3D-printed flexure stages
Sub Title (in English)
Keyword(1) 3D print
Keyword(2) flexure stage
Keyword(3) structured illumination microscope
Keyword(4) SIM
Keyword(5) optical sectioning
Keyword(6) depth resolution
1st Author's Name Tatsunosuke Matsui
1st Author's Affiliation Mie University(Mie Univ.)
2nd Author's Name Daigo Fujiwara
2nd Author's Affiliation Mie University(Mie Univ.)
Date 2022-07-18
Paper # OME2022-17
Volume (vol) vol.122
Number (no) OME-116
Page pp.pp.16-21(OME),
#Pages 6
Date of Issue 2022-07-11 (OME)