| Presentation | 2022-01-25 Effects of ultrasound vibration on differentiation of PC12 cells Haruki Maruyama, Koji Fujiwara, Ryohei Hashiguchi, Daisuke Koyama, Masahiro Kumeta, |
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| PDF Download Page |  PDF download Page Link |
| Abstract(in Japanese) | (See Japanese page) |
| Abstract(in English) | Cell culture techniques for the applications in regenerative medicine have been studied, and our group have been also investigating a technique to control cell culture using ultrasound vibration. The system is comparatively inexpensive and has a simple structure, providing ultrasound vibration to the cells under appropriate culture conditions. In this report, an ultrasound cell culture dish was fabricated, and the effects of ultrasound vibration generated on the bottom of a culture dish to the growth of PC12 cells were examined. It was found that ultrasound vibration promoted growth of the neurites under microscopic observation, and real-time quantitative PCR analysis revealed that the expression levels of the differentiation-related genes of PC12 cell were changed significantly by ultrasound stimulation. |
| Keyword(in Japanese) | (See Japanese page) |
| Keyword(in English) | Cell culture / ultrasound vibration / PC12 cells / nerite / PCR analysis |
| Paper # | US2021-58 |
| Date of Issue | 2022-01-18 (US) |
| Conference Information | |
| Committee | US |
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| Conference Date | 2022/1/25(1days) |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Online |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | High-intensity ultrasound, Ultrasonics, etc. |
| Chair | Yoshikazu Koike(Shibaura Inst. of Tech.) |
| Vice Chair | Hikaru Miura(Nihon Univ.) / Kentaro Nakamura(Tokyo Inst. of Tech.) |
| Secretary | Hikaru Miura(Tohoku Univ.) / Kentaro Nakamura(Chiba Univ.) |
| Assistant | Shin Yoshizawa(Tohoku Univ.) |
| Paper Information | |
| Registration To | Technical Committee on Ultrasonics |
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| Language | JPN |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Effects of ultrasound vibration on differentiation of PC12 cells |
| Sub Title (in English) | |
| Keyword(1) | Cell culture |
| Keyword(2) | ultrasound vibration |
| Keyword(3) | PC12 cells |
| Keyword(4) | nerite |
| Keyword(5) | PCR analysis |
| 1st Author's Name | Haruki Maruyama |
| 1st Author's Affiliation | Doshisha University(Doshisha Univ.) |
| 2nd Author's Name | Koji Fujiwara |
| 2nd Author's Affiliation | Doshisha University(Doshisha Univ.) |
| 3rd Author's Name | Ryohei Hashiguchi |
| 3rd Author's Affiliation | Doshisha University(Doshisha Univ.) |
| 4th Author's Name | Daisuke Koyama |
| 4th Author's Affiliation | Doshisha University(Doshisha Univ.) |
| 5th Author's Name | Masahiro Kumeta |
| 5th Author's Affiliation | Kyoto University(Kyoto Univ.) |
| Date | 2022-01-25 |
| Paper # | US2021-58 |
| Volume (vol) | vol.121 |
| Number (no) | US-348 |
| Page | pp.pp.1-4(US), |
| #Pages | 4 |
| Date of Issue | 2022-01-18 (US) |