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| Presentation | 2019-01-22 10:30 [Poster Presentation] Qualitative evaluation of DNA denaturation using vibration method Keitaro Shimizu, Ryu Kobayashi, Tadzunu Suzuki, Seiji Yoneda, Shigeo Yamaguchi (Kanagawa Univ.)  US2018-79 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | The polymerase chain reaction (PCR) system is an extremely important and well-established technique for DNA amplification, which is widely used in genome sequencing, forensics, and the diagnosis of various diseases. For a successful PCR, precise temperature control is required, and during a PCR process, specimens are subjected to multiple thermal cycles (∼25 cycles) typically at three temperatures: 94, 55, and 72 °C. Transitions between these temperatures, DNA specimens and enzymes are damaged, thus leading to the amplification of nonspecific targets. To overcome this problem, we have proposed and demonstrated a novel amplification method by vibration that does not require high temperature. In this study, we report the results of DNA denatureation and amplification by vibration. |
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| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | DNA / PCR / Vibration / denaturation / Real Time PCR / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 118, no. 409, US2018-79, pp. 7-8, Jan. 2019. | |
| Paper # | US2018-79 | |
| Date of Issue | 2019-01-15 (US) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | US2018-79 |
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| Conference Information | |
| Committee | EA US |
| Conference Date | 2019-01-22 - 2019-01-23 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Doshisha Univ. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | [Joint Meeting on Acoustics and Ultrasonics Subsociety] Engineering/Electro Acoustics, Ultrasonics, and Related Topics |
| Paper Information | |
| Registration To | US |
| Conference Code | 2019-01-EA-US |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Qualitative evaluation of DNA denaturation using vibration method |
| Sub Title (in English) | |
| Keyword(1) | DNA |
| Keyword(2) | PCR |
| Keyword(3) | Vibration |
| Keyword(4) | denaturation |
| Keyword(5) | Real Time PCR |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Keitaro Shimizu |
| 1st Author's Affiliation | Kanagawa University (Kanagawa Univ.) |
| 2nd Author's Name | Ryu Kobayashi |
| 2nd Author's Affiliation | Kanagawa University (Kanagawa Univ.) |
| 3rd Author's Name | Tadzunu Suzuki |
| 3rd Author's Affiliation | Kanagawa University (Kanagawa Univ.) |
| 4th Author's Name | Seiji Yoneda |
| 4th Author's Affiliation | Kanagawa University (Kanagawa Univ.) |
| 5th Author's Name | Shigeo Yamaguchi |
| 5th Author's Affiliation | Kanagawa University (Kanagawa Univ.) |
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| Speaker | Author-1 |
| Date Time | 2019-01-22 10:30:00 |
| Presentation Time | 120 minutes |
| Registration for | US |
| Paper # | US2018-79 |
| Volume (vol) | vol.118 |
| Number (no) | no.409 |
| Page | pp.7-8 |
| #Pages | 2 |
| Date of Issue | 2019-01-15 (US) |
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